Back in the dark ages, we used diisopropylfluorophosphate as a
cholinesterase inhibitor.  It's very effective (and very expensive), but not
very specific: it also inhibits trypsin, thrombin, and plasmin.
Diisopropylfluorophosphate is still available from SIGMA (1-800-325-3010),
cat # D 0879 @ 227.65 / g.
Ethopropazine is still in the current SIGMA catalog, cat # E 2880 @ 16.60 /
5 g.

-----Original Message-----
From: sebres [mailto:sebres@comcast.net] 
Sent: Wednesday, November 05, 2003 6:36 PM
To: Histonet (E-mail)
Subject: Re: [Histonet] ethopropazine/acetylcholinesterase histochemistry


Well, I've already dug a little deeper & come up with part of the answer,
but am still in need of advice:  I understand better now that it is
specifically butyrylcholinesterase that ethopropazine is supposed to
inhibit.  I've found some evidence that ethephon is considered a specific
butyrylcholinesterase inhibitor, & this is actually available for purchase
from agricultural supply companies.  Any thoughts out there on whether this
sounds like a viable solution?   Many thanks,  Susan
----- Original Message ----- 
From: "sebres" 
To: "Histonet (E-mail)" 
Sent: Wednesday, November 05, 2003 6:03 PM
Subject: [Histonet] ethopropazine/acetylcholinesterase histochemistry


> I'm teaching a neurohistology class in a research university, mainly 
> Nissl staining, immunohistochemisty & in situ hybridization 
> histochemistry on
rat
> brain sections.  I thought I'd add to the mix a good old fashioned 
> enzyme histochemistry assay, such as the elegant acetylcholinesterase 
> method described in Paxinos & Watson's Rat Brain atlas, which sounds 
> refreshingly simple.  But, to my shock, ethopropazine, since it is now 
> used medicinally (Parsitan), seems to no longer be available except by 
> prescription!  If I understand this correctly, the main purpose of 
> this reagent in this assay
is
> as a cholinesterase inhibitor, in which case I'm wondering whether it
might
> work to substitute either physostigmine, or possibly haloperidol, both 
> of which I already have in hand?  My students and I would all be extremely
> grateful for any advice about this!     Susan Bachus, George Mason
> University
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
           
                       
                
The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender.  E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses.
Barry University - Miami Shores, FL (http://www.barry.edu) 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet