|From:||"Charles W. Scouten, Ph.D." |
There are many real advantages to a dip and move system (OPEN) when compared to a sealed flush (CLOSED) system. The following points highlight the operational differences 1) Time - the minimum cycle time for a flush system is 2 hours. This is due to the heating phase for the paraffin and the additional rinse cycles. For quick procedures such as biopsies, 2 hours is an inconvenient cycle time when it can be done in 30 minutes with a TPC system. A procedure that would take only 8 hours with the TPC will take 12 hours in a sealed system. 2) Artifacts – In order to introduce the paraffin in a sealed system, the chamber must be heated. This heating process can cause dead spots, shadows, or artifacts in your tissue. The TPC system does not create such artifacts. 3) Maintenance – The TPC system does not rely on pumps to complete the protocols. The sealed system requires pumps which can fail. These can be costly and inconvenient to replace. 4) Contamination – Although the sealed systems are thought to be contamination free, they do require cleaning after every protocol. Proteins are routinely washed out of sections during processing. These proteins will settle in the bottom of flush storage tanks and will be present for future protocols. In order to remove all contaminates, the system must be cleaned. 5) Cost – The TPC system uses a much lower volume of reagents and chemicals. These add up over the coarse of a year. Have you actually calculated your annual costs and looked at the potential savings? 6) Tissue Shrinking – The TPC system does not expose the tissue to a heating cycle and therefore does not cause the tissue to shrink.
I am also attaching a detailed data sheet here. If you have any questions or would like to discuss this further please feel free to contact me anytime.
Charles W. Scouten, Ph.D.
5918 Evergreen Blvd.
St. Louis, MO 63134
Ph: 314 522 0300
FAX 314 522 0377
From: JCarpenter764@aol.com [mailto:JCarpenter764@aol.com]
Sent: Thursday, November 20, 2003 3:10 PM
Subject: [Histonet] different types of processors
Would someone please explain the difference between an open processor and a closed processor....