| From: | "Charles W. Scouten, Ph.D." |
I have responded separately with an attached 7 page discussion of perfusion, and a link to our Perfusion One product. Don’t use flow rate, pressure is what the cardiovascular system controls, use 5% sucrose prewash, no saline, and switch to fixative when the animal stops. Haparin is not needed, all blood can be cleared without it with sufficient pressure.
If any body else would like to see these materials, I will send them.
Charles W. Scouten, Ph.D.
myNeuroLab.com
5918
Evergreen Blvd.
St. Louis,
MO 63134
Ph: 314 522
0300
FAX
314 522 0377
cwscouten@myneurolab.com
www.myneurolab.com
-----Original Message-----
From: Davis, Gareth
[mailto:gareth.davis@Vanderbilt.Edu]
Sent: Saturday, November 15, 2003
3:29 PM
To: Histonet
Subject: [Histonet] Perfusion pump
time
Hello Histonetters,
Recently (within the last couple months) I saw someone post perfusion times for pumps based on the animal being perfused. I tried to find the posting on the Histosearch, but unsuccessful. We are perfusing rats for the olfactory tissue, but would like ideals on the what others have done. Any input would be appreciated, including if heparin is needed in saline, the correct perfusion pump time, and if recirculation is necessary.
thanks,
Gareth
Ms. Gareth B. Davis
Research Assistant II