RE: [Histonet] Not all tissue staining...

From:"Willis, Donna"

We had the same problem and found out the hard way that it was the slides we
were using.  We were using Gold Plus Slides.  They worked great on the Nexes
but when we converted to the Benchmark and XT with the new solutions we had
staining issues.  We sent slides inhouse to Ventana and they researched the
issue.  It wasn't the instruments it was the solutions with the slides.  If
the slides went through Xylene and Alcohols on a automatic stainer there
wasn't a problem but if we put them through the EX Prep and CC1 then the
problem occured.  We went to using Plus Slides and everything is wonderful.
Donna Willis
Histology Lab Manager
Harris Methodist Fort Worth, Tx

-----Original Message-----
From: ANN MARUSKA [mailto:amarusk1@FAIRVIEW.ORG]
Sent: Tuesday, November 04, 2003 12:27 PM
Subject: RE: [Histonet] Not all tissue staining...

I am with you - I would suspect the vortex mixers first.  But perhaps
deparaffinization is a problem.  It is possible that the volume of EZ prep
that goes on the slide - and deparaffinizes - might be the problem....or
could it have been made up improperly......or the line have a bubble in it
that needs to be primed.
I agree that contacting technical service at Ventana would be the first step
in having them walk you thru these procedures and troubleshoot.
I've had my Benchmark for 3 years come this January - and have no
Ann Maruska
Fairview-University Medical Center
Mpls. MN  55454  

>>> "T. Truscott"  11/04/03 11:56AM >>>
Karen, Ventana can be great help, but you can also check that all your 
reagent containers are clean and that LCS didn't get poured into the wrong 
one. Good Luck Tom Truscott USDA-ARS Pullman, WA

>From: "Laurie Colbert" 
>To: "Bauer, Karen" ,"Histonet (E-mail)" 
>Subject: RE: [Histonet] Not all tissue staining...
>Date: Wed, 29 Oct 2003 10:47:26 -0800
>We have four BenchMarks and and a NexES special stainer and whenever we 
>have a problem we call Ventana's customer service.  If they can't 
>troubleshoot over the phone, they are very willing to send a service tech 
>-----Original Message-----
>From: Bauer, Karen []
>Sent: Wednesday, October 29, 2003 10:25 AM
>To: Histonet (E-mail)
>Subject: [Histonet] Not all tissue staining...
>Hello to all,
>There are times when a stained IP slide will have only half of the tissue
>staining positively and not other parts of the tissue, but has the same
>cellularity of the part that is staining. (Did that make sense?)  Same
>tissue cells, but some stain and some do not.  When the Pathologist asks 
>this happens, I can only come up with possible vortex mixing problems or
>that the paraffin did not come out completely.  We've recently switched to
>the BenchMark and this has only happened a few times.  I've checked the
>vortex mixers, and all of the heating pads are working properly.
>Does anyone have any suggestions?
>Thanks in advance.
>Karen L. Bauer HT(ASCP)
>Histology Department
>Luther Hospital
>    ********************Confidentiality  Notice********************
>This message is intended for the sole use of the individual and entity to
>whom it is addressed, and may contain information that is privileged,
>confidential and exempt from disclosure under applicable law.  Any
>unauthorized review, use, disclosure or distribution of this email message,
>including any attachment, is prohibited.  If you are not the intended
>recipient, please advise the sender by reply email and destroy all copies 
>the original message.   Thank you.
>Histonet mailing list
>Histonet mailing list

Frustrated with dial-up? Get high-speed for as low as $26.95.   (Prices may vary by
service area.)

Histonet mailing list



The information transmitted in this e-mail is intended only for

the person or entity to which it is addressed and may contain

confidential and/or privileged material, including "protected

health information."  If you are not the intended recipient,

you are hereby notified that any review, retransmission,

dissemination, distribution, or copying of this message is

strictly prohibited.  If you have received this communication

in error, please destroy and delete this message from any

computer and contact us immediately by return e-mail.



The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law.  If you have received this message in error, you are prohibited from copying, distributing, or using the information.  Please contact the sender immediately by return e-mail and delete the original message from your system.

Histonet mailing list

<< Previous Message | Next Message >>