Leanne,

Without knowing in what context you are wanting to use Hanson's technique, I
would recommend finding a suitable antibody and doing IHC.

From my recollection, the original method of Hanson uses free-floating
frozen sections, and the sections do not withstand the procedure very well.
In addition, consistency is a major problem. The enzyme seems to tolerate
acetone fixation fairly well; aldehyde fixation is only successful in highly
active sites.

There is a gel-method, and a semi-permeable membrane method that are
superior if you must go the route of enzyme histochemistry. Let me know if
you need this info.

Ronnie

Ronnie Houston
Regional Histology Operations Manager
Bon Secours HealthPartners Laboratories
5801 Bremo Road
Richmond, VA 23226
(804) 287 7972
ronnie_houston@bshsi.com


-----Original Message-----
From: leanneharris@eircom.net [mailto:leanneharris@eircom.net]
Sent: Monday, November 17, 2003 9:10 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Hansson's Carbnic Anhydrase.


Dear all,
I wonder can you help me? I am looking for the working protocol for
Hansson's Carbonic Anhydrase as I do not have access to the early edition of
Pearse's Histochemistry. Could anybody please forward me a copy of the
protocol?
Thnaking you in advance,
Leanne.


Leanne Harris,
Dublin Institute of Technology,
School of Biological Sciences,
Kevin Street,
Dublin 8.
Ireland.

00-353-1-4022888
leanneharris@eircom.net

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