RE: [Histonet] Genetics anyone?

From:Philip Oshel

What I get emailing without looking at my references. Feulgen's, yes, 
not periodic acid-Schiff's, although Feulgen's does use Schiff's.
A good reference is Berlyn and Miksche "Botanical Microtechnique and 
Cytochemistry", Chap. 11, pp. 246ff
One of the classic methods.


>He wants a Feulgen stain. Kits are available... take a look at the Scytek or
>Chromavision websites.
>Brett M. Connolly, Ph.D.
>Merck & Co., Inc.
>MRL, Imaging Research
>PO Box 4
>West Point, PA 19486
>PH 215-652-2501
>fax. 215-652-2075
>-----Original Message-----
>From: Cheryl Crowder []
>Sent: Thursday, November 06, 2003 8:47 AM
>To: Histonet
>Subject: [Histonet] Genetics anyone?
>Good morning - This questions was put to me yesterday and, knowing
>little about genetics, I'm stumped.  Can any of you help me.  This
>researcher is collecting samples which he says are either diploid,
>triploid or tetraploid.  He states that someone told him there is a
>stain techniques that "would distinguish the 'ploidy' by the intensity
>of the stained tissue".
>Have any of you heard of such a thing or know someone I can contact for
>this "unusual" question?  Thank you, in advance.  Cheryl
>Cheryl Crowder, BA, HTL(ASCP)
>Chief Technologist
>Anatomic Pathology
>Department of Pathobiological Sciences
>School of Veterinary Medicine
>Louisiana State University
>Skip Bertman Drive
>Baton Rouge, LA  70803
>FAX:  225-578-9720
>Histonet mailing list
>Histonet mailing list

Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison,  WI  53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)

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