Cheryl, As noted by others, ploidy can be determined using the feulgen
stain. While a simple bright-field examination can show differences in stain
intensity related to ploidy that would be a very rough guess as to actual
ploidy differences. More exact analysis requires either an image analysis
system (several on the market) or flow cytometry. 

Here is a concise explanation of one method of ploidy analysis:
http://www.fairimag.co.uk/fairfield/dna-cytometry/ploidy-feulgen.html


Tim Morken
Lab Vision / NeoMarkers
www.labvision.com


-----Original Message-----
From: Cheryl Crowder [mailto:ccrowder@mail.vetmed.lsu.edu] 
Sent: Thursday, November 06, 2003 5:47 AM
To: Histonet
Subject: [Histonet] Genetics anyone?

Good morning - This questions was put to me yesterday and, knowing 
little about genetics, I'm stumped.  Can any of you help me.  This 
researcher is collecting samples which he says are either diploid, 
triploid or tetraploid.  He states that someone told him there is a 
stain techniques that "would distinguish the 'ploidy' by the intensity 
of the stained tissue".  

Have any of you heard of such a thing or know someone I can contact for 
this "unusual" question?  Thank you, in advance.  Cheryl


Cheryl Crowder, BA, HTL(ASCP)
Chief Technologist
Anatomic Pathology
Department of Pathobiological Sciences
School of Veterinary Medicine
Louisiana State University
Skip Bertman Drive
Baton Rouge, LA  70803

225-578-9734
FAX:  225-578-9720


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