RE: [Histonet] Frozen section fixing problems - but paraffin works!

Hello Min-Han Tan

you are not writing which primary antibody you use.
And how you dilute the 0.3 % hydrogen peroxide, in a buffer (like TBS or PBS)  or methanol ?
Just a thought:  0.3 % perox block in buffer for 30 min could be too harsh. The use of methanol, which is no problem in formalin fixed tissue , might destroy the epitope in frozens.


Antje Marcantonio
Novartis Pharma AG
BU Transplantation Research
Basel, Switzerland

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