RE: [Histonet] Frozen section fixing problems - but paraffin works!
From:
antje.marcantonio@pharma.novartis.com
Hello Min-Han Tan
you are not writing which primary antibody you use.
And how you dilute the 0.3 % hydrogen peroxide, in a buffer (like TBS or PBS) or methanol ?
Just a thought: 0.3 % perox block in buffer for 30 min could be too harsh. The use of methanol, which is no problem in formalin fixed tissue , might destroy the epitope in frozens.
Regards,
Antje Marcantonio
Novartis Pharma AG
BU Transplantation Research
Basel, Switzerland