I have done some promising work on mouse tissue using the anti-human DAKO
CD68 PGM1 clone with a mouse on mouse biotinylation detection system.  The
PGM1 clone MO876 labels macrophages and monocytes but not other myeloid
cells.  This may be useful for your work.
Patsy

-----Original Message-----
From: histonet-admin@lists.utsouthwestern.edu
[mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Ramesh
Subrahmanyam
Sent: Wednesday, November 05, 2003 10:46 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Distinguishing monocytes from granulocytes

Hello Histonetters,
I am trying to distinguish immature monocytes from immature granulocytes
in Balb/c mice. Can anyone suggest antibodies for FACS or
immunohistochemistry or RT-PCR markers that would help in this?
Suggestions on other methods would be welcome too.
Thanks in advance,

Ramesh
Brandeis University
Massachusetts


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