Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317

-----Original Message-----
From: brade, brade upland, upland [mailto:newhistotech@yahoo.com]
Sent: Wednesday, November 19, 2003 9:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Biotin block after primary?

Hi,

I accidently added my primary antibody before avidin/biotin blocking.  Is it possible to block after the primary but before a biontinylated secondary?  Thank you for help.

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