RE: [Histonet] Amyloid Precursor Protein

From:Marion Hiles

We use ZYMED BAPP Cat No. 13-0200 at 1:3000 overnight after microwaving in
citrate buffer pH6.

Bob Quilty
Dept of Neuropathology
Frenchay Hospital
Bristol UK

-----Original Message-----
From: Dana Settembre []
Sent: 10 November 2003 16:39
Subject: [Histonet] Amyloid Precursor Protein

Has anyone worked up Amyloid Precursor Protein (APP) on FFPE human
I have NovoCastra's monoclonal antibody.  I have used Alzheimer's
disease brain tissue that works with other antibodies.  I use a very
good Target Retreival solution from DakoCytomation in a steamer for 40
minutes.  I have used it at the recommended dilutions and I have used
them more potent than that too.  Our neuropathologist tells me that it
has been in use regularly for some time now.
Dana Settembre
Immunohistochemistry Lab
Unversity Hospital-UMDNJ
Newark, NJ

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