[Histonet] thin sectioning of PMMA

From:John Baker

Howdy all, Does anyone have a good protocol for getting thin (4-5 
micron) sections from PMMA embedded bone? We use a Riechert-Jung 
Polycut with tungsten knives for sectioning.  A problem in the past 
has been getting the sections to stick to a slide with curling and 
wrinkles being the cause.  Fluorescence of the sections will be 
looked at in future studies.  Thanks for any assistance in this 
matter!  John

Today is the Tomorrow you worried about Yesterday. Was
it worth it?

Birthdays are good for you. Statistics show that the
people who have the most live the longest.

John A. Baker
The University of Michigan
Orthopaedic Research Laboratories
Histology Unit
400 North Ingalls, G160
Ann Arbor, MI 48109-0486
Main lab office phone:734-763-9674
Histology office:734-936-1635

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