[Histonet] polymerization failure _ Technovit 9100New

From:"Tom Schaer"

Dear all:
 
We are having some problems with the polymerization reaction of Technovit 9100New.  Despite keeping things at constant temperatures it fails to polymerize.  We are polymerizing specimens of ovine fem cond with approx 100ml of constituted solution in glass jars.  We have trial vials at 4C, room temp., and -18C and none polymerize.  We used the manufacturer's protocol included in the 9100New kit.
 
Thanks for any insight and help.
 
Best,
 
Tom
--------------------------------------------
Thomas P. Schaer, VMD
Comparative Orthopaedic Research & Tissue Engineering
Department of Clinical Studies
University of Pennsylvania New Bolton Center
382 West Street Road
Kennett Square, PA 19348
tel. 610-444-5800 (x6261 office)
tel. 610-444-5800 (x6131 lab)
fax. 610-925-8100
tpschaer@mail.vet.upenn.edu
 

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