[Histonet] need help for epon
i have embedded thin tissue in epon, after postfixation with osmium
i stuck sections of 5 um on slides with haupt's solution, deplastized in ethoxide of sodium in heat for 30 minutes
and after that stained Goldner's trichrome
tissue was not coloured, and i dont understand why, is it because of osmium ?
pleaze do you have a protocol of Goldner's trichrome especially for epon not for parrafin sections, or another stain to show collagen and mineralized tissu ?
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