[Histonet] immunofluorescence problem
I am doing triple labeling immuno fluorescence of mouse brain tissue and am having some difficulties. My three markers are NeuN, GFAP, and BrdU. Previously when I have triple labeled, I only get gfap and brdu fluorescence and no NeuN (or rarely and inconsistently). I have found out that it is possible that the acid used to denature the DNA for BrdU may be interfering with NeuN antibodies. I was wondering if anyone has also had this problem with NeuN. Does anyone have any ideas to get around the NeuN/hydrochloric acid issue?
Cellular Neurophysiology Lab
University of British Columbia
Vancouver, B.C., Canada
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