[Histonet] ethopropazine/acetylcholinesterase histochemistry


I'm teaching a neurohistology class in a research university, mainly Nissl
staining, immunohistochemisty & in situ hybridization histochemistry on rat
brain sections.  I thought I'd add to the mix a good old fashioned enzyme
histochemistry assay, such as the elegant acetylcholinesterase method
described in Paxinos & Watson's Rat Brain atlas, which sounds refreshingly
simple.  But, to my shock, ethopropazine, since it is now used medicinally
(Parsitan), seems to no longer be available except by prescription!  If I
understand this correctly, the main purpose of this reagent in this assay is
as a cholinesterase inhibitor, in which case I'm wondering whether it might
work to substitute either physostigmine, or possibly haloperidol, both of
which I already have in hand?  My students and I would all be extremely
grateful for any advice about this!     Susan Bachus, George Mason

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