Hello All

 

Is it possible to stain specifically for chondrotin sulfate or hyaluronic acid in the cartilage of decalcified knee joints (guinea pig).  I know that Alcian Blue ph 2.5 will stain for hyaluronic acid.  I do have a previous post from John Kiernan that explains a pretreatment process for the Alcian Blue pH 2.5 to make it specific for hyaluronic acid. But I was wondering if anyone had any other suggestions and what I could do for staining of chondrotin sulfate.

 

Thanks in advance.

 

Liz

 

Elizabeth A. Chlipala, BS, HTL(ASCP)

Premier Histology Laboratory, LLC

P.O. Box 18592

Boulder, Colorado 80308

Office: (303) 735-5001

Fax: (303) 735-3540

lizchlipala@premierhistology.com

www.premierhistology.com

 

Ship to Address:

Premier Histology Laboratory

University of Colorado

MCBD, Room A3B40

Boulder, Colorado 80309

 

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