[Histonet] chondrotin sulfate and hyaluronic acid in decalcifed bone sections

From:"Elizabeth Chlipala"

Hello All


Is it possible to stain specifically for chondrotin sulfate or hyaluronic acid in the cartilage of decalcified knee joints (guinea pig).  I know that Alcian Blue ph 2.5 will stain for hyaluronic acid.  I do have a previous post from John Kiernan that explains a pretreatment process for the Alcian Blue pH 2.5 to make it specific for hyaluronic acid. But I was wondering if anyone had any other suggestions and what I could do for staining of chondrotin sulfate.


Thanks in advance.




Elizabeth A. Chlipala, BS, HTL(ASCP)

Premier Histology Laboratory, LLC

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