[Histonet] Van gieson for frozen sections

From:"Vincenzo La Manna"


Does anyone have tryed before Van Gieson staining on
frozen sections?
I'm working on 8-10 Microns sections, using vectabond
slides. I've found lots of protocols on the net but
all of them refer to wax embedded sections.
I've also found different recipies for the Van Gieson
Stain. Depending from the one you use, you could need
to differentiate after stain with haematoxylin. what
does it mean from the molecular point of view?



-- 
La Manna Vincenzo
PhD Student
Department of Agriculture and Forestry, University of
Aberdeen, Hilton Place block M
AB24 4FA, Aberdeen , UK.
Telephone; 01224 274259
Fax; 01224 273731
e-mail v.lamanna@abdn.ac.uk




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