[Histonet] Unsubscribe

From:"ANNA (Annette Enggaard)"

I would like to unsubscribe to this list

regards Annette Enggaard





-----Original Message-----
From: histonet-request@lists.utsouthwestern.edu
[mailto:histonet-request@lists.utsouthwestern.edu] 
Sent: 5. november 2003 19:00
To: histonet@lists.utsouthwestern.edu
Subject: Histonet digest, Vol 1 #117 - 15 msgs


Send Histonet mailing list submissions to
	histonet@lists.utsouthwestern.edu

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Today's Topics:

   1. Re: Improved Eosin (edmondsj)
   2. RE: couple of questions (Subject line) (Marshall Terry Dr,
Consultant Histopathologist)
   3. RE: RE: Looking for a lab to cut stents (Bernard Ian R SSgt 59
CRES/MSROP)
   4. Re: RE: Looking for a lab to cut stents (Tiffany L Sheffield)
   5. dystrophin (Mcleod)
   6. RE: disposal of tissue (Horn, Hazel V)
   7. RE: disposal of tissue - Formalin Neutralization (Kelly Booher)
   8. RE: disposal of tissue (Claye Clyatt)
   9. RE: disposal of tissue (Laurie Colbert)
  10. Somebody that can do IHC for us??? (NIDAL E MUVARAK)
  11. Re: Histonet digest, Vol 1 #116 - 24 msgs (Instrumedics)
  12. Re: Histonet digest, Vol 1 #116 - 24 msgs (Instrumedics)
  13. RE: couple of questions (Marion Hiles)
  14. Re: Histonet digest, Vol 1 #116 - 24 msgs (Thom Jensen)
  15. Distinguishing monocytes from granulocytes (Ramesh Subrahmanyam)

--__--__--

Message: 1
Date: Wed, 05 Nov 2003 08:51:50 -0500
From: edmondsj 
To: John Difford ,
        Histonet 
Subject: Re: [Histonet] Improved Eosin

John,
I have a Johns Hopkins eosin recipe, it is as follows:

Ingredients:
Eosin Y  18gms
Phloxine B  7.5gms
Biebrich Scarlet  1.5gms
Distilled Water  2850 ml
Absolute Alcohol  750 ml

Procedure:
1: Dissolve all the dyes in the distilled water.
2: Add the absolute alcohol and continue stirring for at least 1
   hour.
This will make up a volume of 3610 ml, I add about two or three drops of

glacial acetic acid to a 500 ml volume before staining tissue (this 
enhances the stain), you may not like the intensity but you can omit the

acetic acid if you like.  I hope this is helpful to you.

Joyce Edmonds
Research Specialist
Medical University of SC
Charleston, SC

--On Tuesday, November 04, 2003 5:02 PM -0500 John Difford 
 wrote:

>
> Dear All,
>
> Does anyone remember a formula for an improved Eosin solution which 
> was displayed on Histonet several years ago?
>
> It was said to have orginated at Johns Hopkins and contained some 
> other red dye in addition to eosin.
>
> I want to make up some more because it was very good for frozen 
> sections, but I have lost the instructions!
>
> Many thanks
>
> John Difford
> Histopathology Dept
> Royal Free Hospital
> London
> England, UK
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet




--__--__--

Message: 2
Date: Wed, 5 Nov 2003 14:02:27 -0000
From: "Marshall Terry Dr, Consultant Histopathologist"

To: "Marshall Terry Dr, Consultant Histopathologist"
,
	,
	
Subject: RE: [Histonet] couple of questions (Subject line)

A more garbled incoherent load of rubbish is hard to imagine. Who is
this T Marshall anyway?

Sorry, tried to do 2 things at once. Wrong sex.

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path  Consultant
Pathologist  Rotherham General Hospital  South Yorkshire  England
        terry.marshall@rothgen.nhs.uk

-----Original Message-----
From: Marshall Terry Dr, Consultant Histopathologist=20
Sent: 05 November 2003 13:45
To: jkiernan@uwo.ca; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] couple of questions (Subject line)


Received 2 days ago:

Re: [Histonet] (no subject)

"You, a (? new) histonetter sent a message with=20
the header:  couple of questions

Grrrr! =20

The Subject line is the most important part of
every email in these days of 2 much XS spam.

PLEASE put a few specific words in the
Subject line. "

Rremeber who sent it John:-)

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path  Consultant
Pathologist  Rotherham General Hospital  South Yorkshire  England
        terry.marshall@rothgen.nhs.uk

-----Original Message-----
From: John Kiernan [mailto:jkiernan@uwo.ca]
Sent: 05 November 2003 06:10
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] couple of questions (Subject line)


Dear *********

You, a (? new) histonetter sent a message with=20
the header:  couple of questions

Grrrr! =20

The Subject line is the most important part of
every email in these days of 2 much XS spam.

PLEASE put a few specific words in the
Subject line.  Your email asked two questions
(another not good thing).  For best replies,
try sending two Histonet queries, with=20
different Subject lines such as:

Hoovering the cryostat safely

Solvents and the VIP processor

I've obscured your identity in this Histonet
reply because It's not intended to be "flaming."
There are lots of emails with no-good subject
lines. I'm just trying to ask everyone to
send a pertinent Subject line above every=20
message.

We all get lots of junk email, and we delete
most of the junk messages on the basis of a
few word in the Subject line. Herb and Linda=20
are doing a SUPERB job protecting Histonet
subscribers from all the viagra adverts etc. =20
--=20
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan@uwo.ca
   http://publish.uwo.ca/~jkiernan/ ______________________________

She Who Shall Not be Named wrote:
>=20
> Hi Everyone,
> I have a few questions,
> 1. Is anyone using a "home" type vacuum for the
> cryostat. Of course the bags would go in the medical
> waste.
> 2. Is Alcoholic formalin OK to put on the VIP tissue  processor, there

>is no acetic acid in the solution. =20
> I appreciate all your help.
_______________________________________

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


--__--__--

Message: 3
From: Bernard Ian R SSgt 59 CRES/MSROP 
To: "'Gordon Grant'" ,
histonet@lists.utsouthwestern.edu
Date: Wed, 5 Nov 2003 08:07:24 -0600 
Subject: RE: [Histonet] RE: Looking for a lab to cut stents

Cathy MAYton in Arizona Does this.

-----Original Message-----
From: Gordon Grant [mailto:grantgd@comcast.net]
Sent: Tuesday, November 04, 2003 5:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Looking for a lab to cut stents


I was wondering if someone out there can give me a recommendation for a
lab which can provide good quality sections of implanted stents.
Specifically, we have been told the FDA likes the thick saw cut, ground
and polished sections rather than microtomed sections.  

Thanks Gordon Grant

-----Original Message-----
From: histonet-admin@lists.utsouthwestern.edu
[mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of
histonet-request@lists.utsouthwestern.edu
Sent: Sunday, November 02, 2003 10:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet digest, Vol 1 #113 - 2 msgs

Send Histonet mailing list submissions to
	histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
	http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
	histonet-request@lists.utsouthwestern.edu

You can reach the person managing the list at
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When replying, please edit your Subject line so it is more specific than
"Re: Contents of Histonet digest..."


Today's Topics:

   1. appropriate pay for grossing techs (Jen Steinberg)
   2. Re: appropriate pay for grossing techs (Gudrun Lang)

-- __--__-- 

Message: 1
Date: Sat, 1 Nov 2003 13:32:39 -0800 (PST)
From: Jen Steinberg 
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] appropriate pay for grossing techs

--0-1595893577-1067722359=:57919
Content-Type: text/plain; charset=us-ascii

Hi,
   I'm hoping someone can help me.  I was wondering what the appropriate
starting wage of a grossing tech would be for a private path lab which
works mainly on small skin, gyn, and oral specimens and processes about
250 cases a day (between 2 grossers).  Would the pay be any different if
the employee had an M.S.  The position is entry-level.  Thanks very
much.  
 
 


---------------------------------
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Exclusive Video Premiere - Britney Spears
--0-1595893577-1067722359=:57919
Content-Type: text/html; charset=us-ascii

Hi,
   I'm hoping someone can help me.  I was wondering what the appropriate starting wage of a grossing tech would be for a private path lab which works mainly on small skin, gyn, and oral specimens and processes about 250 cases a day (between 2 grossers).  Would the pay be any different if the employee had an M.S.  The position is entry-level.  Thanks very much. 
 
 


Do you Yahoo!?
Exclusive Video Premiere - Britney Spears --0-1595893577-1067722359=:57919-- -- __--__-- Message: 2 From: "Gudrun Lang" To: "Histonetliste" , "Jen Steinberg" Date: Sun, 2 Nov 2003 13:19:09 +0100 Subject: Re: [Histonet] appropriate pay for grossing techs This is a multi-part message in MIME format. ------=_NextPart_000_001B_01C3A143.E6310980 Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable Hi Jen, I dont have an answer for you, but another question. In Austria the = person, who does crossing, is a pathologist (m.d.).=20 We have several sorts of tissue f(rom toe to sculp) and I think without = a medical uni-study, it is very difficult. Even our doctors sometimes = make mistakes and look over important details. Is it usual in USA, that histotechs do the crossing? best wishes Gudrun Lang general hospital Linz, Austria ----- Original Message -----=20 From: Jen Steinberg=20 To: histonet@lists.utsouthwestern.edu=20 Sent: Saturday, November 01, 2003 10:32 PM Subject: [Histonet] appropriate pay for grossing techs Hi, I'm hoping someone can help me. I was wondering what the = appropriate starting wage of a grossing tech would be for a private path = lab which works mainly on small skin, gyn, and oral specimens and =3D processes about 250 cases a day (between 2 grossers). Would the pay be = any different if the employee had an M.S. The position is entry-level. = Thanks very much. =20 ------------------------------------------------------------------------ -= ----- Do you Yahoo!? Exclusive Video Premiere - Britney Spears ------=_NextPart_000_001B_01C3A143.E6310980 Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable
Hi Jen,
I dont have an answer for you, but = another=20 question. In Austria the person, who does crossing, is a pathologist = (m.d.).=20
We have several sorts of tissue f(rom = toe to sculp)=20 and I think without a medical uni-study, it is very difficult. Even our = doctors=20 sometimes make mistakes and look over important details.
Is it usual in USA,  that = histotechs do=20 the crossing?
 
best wishes
Gudrun Lang
general hospital Linz, = Austria
----- Original Message -----
From:=20 Jen=20 Steinberg
To: histonet@lists.utsouth w= estern.edu=20
Sent: Saturday, November 01, =3D 2003 10:32=20 PM
Subject: [Histonet] appropriate = pay for=20 grossing techs

Hi,
   I'm hoping someone can help me.  I was = wondering what=20 the appropriate starting wage of a grossing tech would be for a = private path=20 lab which works mainly on small skin, gyn, and oral specimens and = processes=20 about 250 cases a day (between 2 grossers).  Would the pay be any = different if the employee had an M.S.  The position is = entry-level. =20 Thanks very much. 
 
 


Do you Yahoo!?
Exclusive Video Premiere - Britney=20 Spears ------=_NextPart_000_001B_01C3A143.E6310980-- -- __--__-- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --__--__-- Message: 4 Date: Wed, 05 Nov 2003 08:14:38 -0600 From: "Tiffany L Sheffield" To: Bernard Ian R SSgt 59 CRES/MSROP CC: 'Gordon Grant' , histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Looking for a lab to cut stents This is a multi-part message in MIME format. --------------2B9EB8314574D12854D86640 Content-Type: multipart/alternative; boundary="------------4B5728D5400BFA6100977CDE" --------------4B5728D5400BFA6100977CDE Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit My lab does stents as well in Houston! We work with Texas Heart Institute in conjunction with other companies on a regular basis. We do thick saw cut ground and polished sections. Hope this helps! If you have any further questions feel free to contact me. Tiffany:) Bernard Ian R SSgt 59 CRES/MSROP wrote: > Cathy MAYton in Arizona Does this. > > -----Original Message----- > From: Gordon Grant [mailto:grantgd@comcast.net] > Sent: Tuesday, November 04, 2003 5:16 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Looking for a lab to cut stents > > I was wondering if someone out there can give me a recommendation for > a lab which can provide good quality sections of implanted stents. > Specifically, we have been told the FDA likes the thick saw cut, > ground and polished sections rather than microtomed sections. > > Thanks Gordon Grant > > -----Original Message----- > From: histonet-admin@lists.utsouthwestern.edu > [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of > histonet-request@lists.utsouthwestern.edu > Sent: Sunday, November 02, 2003 10:00 AM > To: histonet@lists.utsouthwestern.edu > Subject: Histonet digest, Vol 1 #113 - 2 msgs > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-admin@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > Today's Topics: > > 1. appropriate pay for grossing techs (Jen Steinberg) > 2. Re: appropriate pay for grossing techs (Gudrun Lang) > > -- __--__-- > > Message: 1 > Date: Sat, 1 Nov 2003 13:32:39 -0800 (PST) > From: Jen Steinberg > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] appropriate pay for grossing techs > > --0-1595893577-1067722359=:57919 > Content-Type: text/plain; charset=us-ascii > > Hi, > I'm hoping someone can help me. I was wondering what the > appropriate starting wage of a grossing tech would be for a private > path lab which works mainly on small skin, gyn, and oral specimens and > processes about 250 cases a day (between 2 grossers). Would the pay > be any different if the employee had an M.S. The position is > entry-level. Thanks very much. > > > > --------------------------------- > Do you Yahoo!? > Exclusive Video Premiere - Britney Spears > --0-1595893577-1067722359=:57919 > Content-Type: text/html; charset=us-ascii > >
Hi,
>
   I'm hoping someone can help me.  I was > wondering what the appropriate starting wage of a grossing tech would > be for a private path lab which works mainly on small skin, gyn, and > oral specimens and processes about 250 cases a day (between 2 > grossers).  Would the pay be any different if the employee had an > M.S.  The position is entry-level.  Thanks very much.  >
 
>
 


> Do you Yahoo!?
> Exclusive Video Premiere - href="http://launch.yahoo.com/video/?1093432&fs=1&redirectURL=http://lau > nch.yahoo.com/promos/britneyspears/">Britney Spears > --0-1595893577-1067722359=:57919-- > > -- __--__-- > > Message: 2 > From: "Gudrun Lang" > To: "Histonetliste" , > "Jen Steinberg" > Date: Sun, 2 Nov 2003 13:19:09 +0100 > Subject: Re: [Histonet] appropriate pay for grossing techs > > This is a multi-part message in MIME format. > > ------=_NextPart_000_001B_01C3A143.E6310980 > Content-Type: text/plain; > charset="iso-8859-1" > Content-Transfer-Encoding: quoted-printable > > Hi Jen, > I dont have an answer for you, but another question. In Austria the =3D > person, who does crossing, is a pathologist (m.d.).=20 We have several > sorts of tissue f(rom toe to sculp) and I think without = > a medical uni-study, it is very difficult. Even our doctors sometimes = > make mistakes and look over important details. > Is it usual in USA, that histotechs do the crossing? > > best wishes > Gudrun Lang > general hospital Linz, Austria > ----- Original Message -----=20 > From: Jen Steinberg=20 > To: histonet@lists.utsouthwestern.edu=20 > Sent: Saturday, November 01, 2003 10:32 PM > Subject: [Histonet] appropriate pay for grossing techs > > Hi, > I'm hoping someone can help me. I was wondering what the = > appropriate starting wage of a grossing tech would be for a private > path = lab which works mainly on small skin, gyn, and oral specimens > and = processes about 250 cases a day (between 2 grossers). Would the > pay be = > any different if the employee had an M.S. The position is entry-level. > = > Thanks very much. =20 > > ---------------------------------------------------------------------- > -- > -= > ----- > Do you Yahoo!? > Exclusive Video Premiere - Britney Spears > > ------=_NextPart_000_001B_01C3A143.E6310980 > Content-Type: text/html; > charset="iso-8859-1" > Content-Transfer-Encoding: quoted-printable > > > charset=3Diso-8859-1"> > > > > >
Hi Jen,
>
I dont have an answer for you, but = > another=20 > question. In Austria the person, who does crossing, is a pathologist =3D > (m.d.).=20 >
>
We have several sorts of tissue f(rom = > toe to sculp)=20 > and I think without a medical uni-study, it is very difficult. Even our > = > doctors=20 > sometimes make mistakes and look over important details.
>
Is it usual in USA,  that = > histotechs do=20 > the crossing?
>
 
>
best wishes
>
Gudrun Lang
>
general hospital Linz, = > Austria
> style=3D"PADDING-RIGHT: 0px; PADDING-LEFT: 5px; MARGIN-LEFT: 5px; =3D > BORDER-LEFT: #000000 2px solid; MARGIN-RIGHT: 0px"> >
----- Original Message -----
> style=3D"BACKGROUND: #e4e4e4; FONT: 10pt arial; font-color: = > black">From:=20 > href=3D"mailto:pathologygrl@yahoo.com">Jen=20 > Steinberg >
To: title=3Dhistonet@lists.utsouthwestern.edu=20 > = > href=3D"mailto:histonet@lists.utsouthwestern.edu">histonet@lists.utsouth > w= > estern.edu=20 >
>
Sent: Saturday, November 01, = > 2003 10:32=20 > PM
>
Subject: [Histonet] appropriate > = > pay for=20 > grossing techs
>

>
Hi,
>
   I'm hoping someone can help me.  I was = > wondering what=20 > the appropriate starting wage of a grossing tech would be for a = > private path=20 > lab which works mainly on small skin, gyn, and oral specimens and =3D > processes=20 > about 250 cases a day (between 2 grossers).  Would the pay be any > = > > different if the employee had an M.S.  The position is = > entry-level. =20 > Thanks very much. 
>
 
>
 
>

>


> Do you Yahoo!?
Exclusive Video Premiere - = > href=3D"http://launch.yahoo.com/video/?1093432&fs=3D1&redirect > UR > L= > =3Dhttp://launch.yahoo.com/promos/britneyspears/">Britney=20 > Spears > > ------=_NextPart_000_001B_01C3A143.E6310980-- > > -- __--__-- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------4B5728D5400BFA6100977CDE Content-Type: text/html; charset=us-ascii Content-Transfer-Encoding: 7bit My lab does stents as well in Houston! We work with Texas Heart Institute in conjunction with other companies on a regular basis. We do thick saw cut ground and polished sections.
Hope this helps!
If you have any further questions feel free to contact me.
Tiffany:)

Bernard Ian R SSgt 59 CRES/MSROP wrote:

Cathy MAYton in Arizona Does this.

-----Original Message-----
From: Gordon Grant [mailto:grantgd@comcast.net]
Sent: Tuesday, November 04, 2003 5:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Looking for a lab to cut stents

I was wondering if someone out there can give me a recommendation for a
lab which can provide good quality sections of implanted stents.
Specifically, we have been told the FDA likes the thick saw cut, ground
and polished sections rather than microtomed sections.

Thanks Gordon Grant

-----Original Message-----
From: histonet-admin@lists.utsouthwestern.edu
[mailto:histonet-ad min@lists.utsouthwestern.edu] On Behalf Of
histonet-request@lists.utsouthwestern.edu
Sent: Sunday, November 02, 2003 10:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet digest, Vol 1 #113 - 2 msgs

Send Histonet mailing list submissions to
        histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
        http:// lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
        histonet-request@lists.utsouthwestern.edu

You can reach the person managing the list at
        histonet-admin@lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."

Today's Topics:

   1. appropriate pay for grossing techs (Jen Steinberg)
   2. Re: appropriate pay for grossing techs (Gudrun Lang)

-- __--__--

Message: 1
Date: Sat, 1 Nov 2003 13:32:39 -0800 (PST)
From: Jen Steinberg <pathologygrl@yahoo.com>
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] appropriate pay for grossing techs

--0-1595893577-1067722359=:57919
Content-Type: text/plain; charset=us-ascii

Hi,
   I'm hoping someone can help me.  I was wondering what the appropriate
starting wage of a grossing tech would be for a private path lab which
works mainly on small skin, gyn, and oral specimens and processes about
250 cases a day (between 2 grossers).  Would the pay be any different if
the employee had an M.S.  The position is entry-level.  Thanks very
much.
 
 

---------------------------------
Do you Yahoo!?
Exclusive Video Premiere - Britney Spears
--0-1595893577-1067722359=:57919
Content-Type: text/html; charset=us-ascii

<DIV>Hi,</DIV>
<DIV>&nbsp;&nbsp; I'm hoping someone can help me.&nbsp; I was wondering
what the appropriate starting wage of a grossing tech would be for a
private path lab which works mainly on small skin, gyn, and oral
specimens and processes about 250 cases a day (between 2
grossers).&nbsp; Would the pay be any different if the employee had an
M.S.&nbsp; The position is entry-level.&nbsp; Thanks very much.&nbsp;
</DIV>
<DIV>&nbsp;</DIV>
<DIV>&nbsp;</DIV><p><hr SIZE=1>
Do you Yahoo!?<br>
Exclusive Video Premiere - <a
href="http://launch.yahoo.com/video/?1093432&fs=1&redirectURL=http:/ /lau
nch.yahoo.com/promos/britneyspears/">Britney Spears</a>
--0-1595893577-1067722359=:57919--

-- __--__--

Message: 2
From: "Gudrun Lang" <gudrun.lang@aon.at>
To: "Histonetliste" <histonet@lists.utsouthwestern.edu>,
        "Jen Steinberg" <pathologygrl@yahoo.com>
Date: Sun, 2 Nov 2003 13:19:09 +0100
Subject: Re: [Histonet] appropriate pay for grossing techs

This is a multi-part message in MIME format.

------=_NextPart_000_001B_01C3A143.E6310980
Content-Type: text/plain;
        charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable

Hi Jen,
I dont have an answer for you, but another question. In Austria the =
person, who does crossing, is a pathologist (m.d.).=20
We have several sorts of tissue f(rom toe to sculp) and I think without
=
a medical uni-study, it is very difficult. Even our doctors sometimes =
make mistakes and look over important details.
Is it usual in USA,  that histotechs do the crossing?

best wishes
Gudrun Lang
general hospital Linz, Austria
  ----- Original Message -----=20
  From: Jen Steinberg=20
  To: histonet@lists.utsouthwestern.edu=20
  Sent: Saturday, November 01, 2003 10:32 PM
  Subject: [Histonet] appropriate pay for grossing techs

  Hi,
     I'm hoping someone can help me.  I was wondering what the =
appropriate starting wage of a grossing tech would be for a private path
=
lab which works mainly on small skin, gyn, and oral specimens and =
processes about 250 cases a day (between 2 grossers).  Would the pay be
=
any different if the employee had an M.S.  The position is entry-level.
=
Thanks very much. =20

--------------------------------------------------------------------- ---
-=
-----
  Do you Yahoo!?
  Exclusive Video Premiere - Britney Spears

------=_NextPart_000_001B_01C3A143.E6310980
Content-Type: text/html;
        charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable

<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN">
<HTML><HEAD>
<META http-equiv=3DContent-Type content=3D"text/html; =
charset=3Diso-8859-1">
<META content=3D"MSHTML 5.50.4807.2300" name=3DGENERATOR>
<STYLE></STYLE>
</HEAD>
<BODY bgColor=3D#ffffff>
<DIV><FONT face=3DArial size=3D2>Hi Jen,</FONT></DIV>
<DIV><FONT face=3DArial size=3D2>I dont have an answer for you, but =
another=20
question. In Austria the person, who does crossing, is a pathologist =
(m.d.).=20
</FONT></DIV>
<DIV><FONT face=3DArial size=3D2>We have several sorts of tissue f(rom =
toe to sculp)=20
and I think without a medical uni-study, it is very difficult. Even our
=
doctors=20
sometimes make mistakes and look over important details.</FONT></DIV>
<DIV><FONT face=3DArial size=3D2>Is it usual in&nbsp;USA, &nbsp;that =
histotechs do=20
the crossing?</FONT></DIV>
<DIV>&nbsp;</DIV>
<DIV><FONT face=3DArial size=3D2>best wishes</FONT></DIV>
<DIV><FONT face=3DArial size=3D2>Gudrun Lang</FONT></DIV>
<DIV><FONT face=3DArial size=3D2>general hospital Linz, =3D
Austria</FONT></DIV>
<BLOCKQUOTE=20
style=3D"PADDING-RIGHT: 0px; PADDING-LEFT: 5px; MARGIN-LEFT: 5px; =3D
BORDER-LEFT: #000000 2px solid; MARGIN-RIGHT: 0px">
  <DIV style=3D"FONT: 10pt arial">----- Original Message ----- </DIV>
  <DIV=20
  style=3D"BACKGROUND: #e4e4e4; FONT: 10pt arial; font-color: =
black"><B>From:</B>=20
  <A title=3Dpathologygrl@yahoo.com =
href=3D"mailto:pathologygrl@yahoo.com"> Jen=20
  Steinberg</A> </DIV>
  <DIV style=3D"FONT: 10pt arial"><B>To:</B> <A=20
  title=3Dhistonet@lists.utsouthwestern.edu=20
  =
href=3D"mailto:histonet@lists.ut southwestern.edu">histonet@lists.utsouth
w=
estern.edu</A>=20
  </DIV>
  <DIV style=3D"FONT: 10pt arial"><B>Sent:</B> Saturday, November 01, =
2003 10:32=20
  PM</DIV>
  <DIV style=3D"FONT: 10pt arial"><B>Subject:</B> [Histonet] appropriate
=
pay for=20
  grossing techs</DIV>
  <DIV><BR></DIV>
  <DIV>Hi,</DIV>
  <DIV>&nbsp;&nbsp; I'm hoping someone can help me.&nbsp; I was =
wondering what=20
  the appropriate starting wage of a grossing tech would be for a =
private path=20
  lab which works mainly on small skin, gyn, and oral specimens and =
processes=20
  about 250 cases a day (between 2 grossers).&nbsp; Would the pay be any
=

  different if the employee had an M.S.&nbsp; The position is =
entry-level.&nbsp;=20
  Thanks very much.&nbsp; </DIV>
  <DIV>&nbsp;</DIV>
  <DIV>&nbsp;</DIV>
  <P>
  <HR SIZE=3D1>
  Do you Yahoo!?<BR>Exclusive Video Premiere - <A=20
  =
href=3D" http://launch.yahoo.com/video/?1093432&amp;fs=3D1&amp;redirectUR
L=
=3Dhttp://launch.yahoo.com/promos/britneyspears/">Britney=20
  Spears</A></BLOCKQUOTE></BODY></HTML>

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--------------4B5728D5400BFA6100977CDE-- --------------2B9EB8314574D12854D86640 Content-Type: text/x-vcard; charset=us-ascii; name="Tiffany.L.Sheffield.vcf" Content-Transfer-Encoding: 7bit Content-Description: Card for Tiffany Sheffield-Lopez Content-Disposition: attachment; filename="Tiffany.L.Sheffield.vcf" begin:vcard n:Sheffield-Lopez;Tiffany tel;fax:713-500-0729 tel;work:713-500-6803 x-mozilla-html:FALSE org:UT Houston Medical School;Orthopaedic Surgery adr:;;6431 Fannin Street, MSB 6.143;Houston ;TX;77030; version:2.1 email;internet:Tiffany.L.Sheffield@uth.tmc.edu title:Supervisor, Bone Histomorphometry & Biomaterials Lab fn:Tiffany Sheffield-Lopez, HT(ASCP) end:vcard --------------2B9EB8314574D12854D86640-- --__--__-- Message: 5 Date: Wed, 05 Nov 2003 16:41:34 +0200 From: Mcleod Reply-To: hmcleod@chempath.uct.ac.za Organization: University of Cape Town To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] dystrophin Thankyou to all who replied to my dystrophin query. Much appreciated. Heather McLeod. --__--__-- Message: 6 From: "Horn, Hazel V" To: 'Diana McCaig' , histonet@lists.utsouthwestern.edu Date: Wed, 5 Nov 2003 08:45:38 -0600 Subject: RE: [Histonet] disposal of tissue Our disposal company requires us to empty the formalin from all the containers. We then collect the formalin and it is also taken away for disposal. I'm wondering what neutralizers anyone is using for formalin so we could dispose of it down the drain?? Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 -----Original Message----- From: Diana McCaig [mailto:dmccaig@ckha.on.ca] Sent: Tuesday, November 04, 2003 2:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] disposal of tissue How do labs dispose of pathological waste when incineration is not available on site. Do you have to empty each individual 90 ml container or will companies take them away with the fluid still in them? I realize most larger containers are drained and the containers recycled but was curious to see what is done with the smaller containers. How long are the containers retained and is this process done by lab staff in the histology department. Diana McCaig, R.T. Charge Tech, Histology Chatham Kent Health Alliance 519-352-6401 (3347) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Arkansas Children's Hospital --__--__-- Message: 7 Date: Wed, 5 Nov 2003 07:05:01 -0800 (PST) From: Kelly Booher To: "Horn, Hazel V" Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] disposal of tissue - Formalin Neutralization We neutralize our formalin with Sakura's Tissue-Tek Neutralex. The reaction takes approximately 15 minutes, and then the neutralized formalin is ready to go down the drain. Kelly Booher, HTL (ASCP) Anatomic Pathology Swedish Medical Center, Providence Campus Seattle, WA 98122 --- "Horn, Hazel V" wrote: > Our disposal company requires us to empty the > formalin from all the > containers. We then collect the formalin and it is > also taken away for > disposal. I'm wondering what neutralizers anyone > is using for formalin so > we could dispose of it down the drain?? > > Hazel Horn, HT/HTL (ASCP) > Histology Supervisor > Arkansas Children's Hospital > > Phone - 501.364.4240 > Fax - 501.364.3912 > > -----Original Message----- > From: Diana McCaig [mailto:dmccaig@ckha.on.ca] > Sent: Tuesday, November 04, 2003 2:22 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] disposal of tissue > > > How do labs dispose of pathological waste when > incineration is not available > on site. Do you have to empty each individual 90 ml container or will > companies take them away with the fluid still in > them? I realize most > larger containers are drained and the containers > recycled but was curious to > see what is done with the smaller containers. How > long are the containers > retained and is this process done by lab staff in > the histology department. > Diana McCaig, R.T. > Charge Tech, Histology > Chatham Kent Health Alliance > 519-352-6401 (3347) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information contained in this message may be > privileged and confidential and protected from > disclosure. If the reader of this message is not the > intended recipient, or an employee or agent > responsible for delivering this message to the > intended recipient, you are hereby notified that any dissemination, > distribution or copying of this communication is strictly prohibited. > If you have received this communication in error, please notify > us immediately by replying to the message and > deleting it from your computer. Thank you. Arkansas > Children's Hospital > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________ Do you Yahoo!? Protect your identity with Yahoo! Mail AddressGuard http://antispam.yahoo.com/whatsnewfree --__--__-- Message: 8 Date: Wed, 05 Nov 2003 11:03:37 -0500 From: "Claye Clyatt" To: ,, Subject: RE: [Histonet] disposal of tissue FRC-5 available from S & S Co of GA (912-435-8394). Claye Claye Clyatt Chief Histotechnologist Department of Pathology=20 Room #BF119 Medical College of Georgia Augusta, Ga 30912 office (706) 721-3630 pager (706) 721-7243-1132 e-mail: cclyatt@mail.mcg.edu >>> "Horn, Hazel V" 11/05/03 09:45AM >>> Our disposal company requires us to empty the formalin from all the containers. We then collect the formalin and it is also taken away for disposal. I'm wondering what neutralizers anyone is using for formalin = so we could dispose of it down the drain?? Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912=20 -----Original Message----- From: Diana McCaig [mailto:dmccaig@ckha.on.ca]=20 Sent: Tuesday, November 04, 2003 2:22 PM To: histonet@lists.utsouthwestern.edu=20 Subject: [Histonet] disposal of tissue How do labs dispose of pathological waste when incineration is not = available on site. Do you have to empty each individual 90 ml container or will companies take them away with the fluid still in them? I realize most larger containers are drained and the containers recycled but was curious = to see what is done with the smaller containers. How long are the containers retained and is this process done by lab staff in the histology department.= Diana McCaig, R.T.=20 Charge Tech, Histology Chatham Kent Health Alliance 519-352-6401 (3347) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu=20 http://lists.utsouthwestern.edu/mailman/listinfo/histonet=20 The information contained in this message may be privileged and confidentia= l and protected from disclosure. If the reader of this message is not the = intended recipient, or an employee or agent responsible for delivering = this message to the intended recipient, you are hereby notified that any = dissemination, distribution or copying of this communication is strictly = prohibited. If you have received this communication in error, please = notify us immediately by replying to the message and deleting it from your = computer. Thank you. Arkansas Children's Hospital=20 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu=20 http://lists.utsouthwestern.edu/mailman/listinfo/histonet --__--__-- Message: 9 Date: Wed, 5 Nov 2003 08:15:40 -0800 From: "Laurie Colbert" To: "Diana McCaig" , "Histonet (E-mail)" Subject: RE: [Histonet] disposal of tissue We have a hazardous waste company that comes out and dumps all of our =3D specimens for us. He separates the formalin from the specimens in our =3D Morgue and takes both for disposal. He has all of the proper PPE, = including a respirator. It is expensive, but I feel it is worth every =3D penny. The time it takes to do the job and the cost of the neutralizer = is expensive, too. If ventilation is not adequate and the formalin =3D exposure is too high for the employees to perform this procedure safely, = they would have to wear a respirator, and this requires formal = respirator training and fitting. I know there is another company here in southern California that takes =3D all of the specimens with the formalin still on them. I don't know if =3D they separate them out later or just burn everything. Laurie Colbert Huntington Hospital Pasadena, CA=20 -----Original Message----- From: Diana McCaig [mailto:dmccaig@ckha.on.ca] Sent: Tuesday, November 04, 2003 12:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] disposal of tissue How do labs dispose of pathological waste when incineration is not = available on site. Do you have to empty each individual 90 ml container or will companies take them away with the fluid still in them? I realize most larger containers are drained and the containers recycled but was = curious to see what is done with the smaller containers. How long are the = containers retained and is this process done by lab staff in the histology = department. Diana McCaig, R.T.=20 Charge Tech, Histology Chatham Kent Health Alliance 519-352-6401 (3347) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --__--__-- Message: 10 Date: Wed, 05 Nov 2003 10:33:05 -0600 From: NIDAL E MUVARAK To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Somebody that can do IHC for us??? Hi. I was wondering if there's a lab(s) out there that can do immunos for our lab. We just need help with MMP-2 and MMP-9, I got everything else to work except for these two and I'm getting frustrated. So if anyone knows of a place that provides that, I'd be very grateful if I can get some information on how to get a hold of those people. Thank you for your time. Nidal E Muvarak Associate Research Specialist Vascular Tissue Biomechanics Laboratory Department of Biomedical Engineering University of Wisconsin-Madison 1550 Engineering Dr.; Rm. 2158 Madison, WI 53706-1609 Lab: (608) 265-8921; Office: (608) 265-4205; Home: (608) 256-7934; Cell: (608) 332-6068 http://vtb.bme.wisc.edu --__--__-- Message: 11 Reply-To: "Instrumedics" From: "Instrumedics" To: Date: Wed, 5 Nov 2003 11:50:10 -0500 Subject: [Histonet] Re: Histonet digest, Vol 1 #116 - 24 msgs Julian, Do you need an instrument for making the recipient block? If so most labs use the Beecher Arrayer. We market the Paraffin Tape-Transfer system that many labs find very valuable for cutting the section of cores and placing them on a slide. The tape-transfer method eliminates the water bath where cores often are lost. Please visit our web site for details www.instrumedics.com . If you need any further information please contact us. Bernice schiller@instrumedics.com --__--__-- Message: 12 Reply-To: "Instrumedics" From: "Instrumedics" To: Date: Wed, 5 Nov 2003 12:00:03 -0500 Subject: [Histonet] Re: Histonet digest, Vol 1 #116 - 24 msgs Eileen, The problem with using the "home" vacuum for cleaning a cryostat is that the debris collected melts and clogs the vacuum bag causing the suctioning to fail. We designed the Cryo-Vac-Away to avoid the clogging problem. The debris that is suctioned as it is generated at the block face is collected in a cold filter that is INSIDE the cryostat. The debris freeze dries and becomes very porous so that the vacuum continues to function effectively. We also have a viral/bacterial filter downstream of the primary filter that will capture pathogens so that the air that exits the system is free of toxins. Bernice schiller@instrumedics.com --__--__-- Message: 13 From: Marion Hiles To: 'eileen dusek' Cc: "Histonet (E-mail)" Date: Wed, 5 Nov 2003 16:56:27 -0000 Subject: RE: [Histonet] couple of questions This message is in MIME format. Since your mail reader does not understand this format, some or all of this message may not be legible. ------_=_NextPart_001_01C3A3BD.C0D3779A Content-Type: text/plain; charset="iso-8859-1" Answer to question 2: Why would it not be OK to use alcoholic formalin since both, as separate solutions, are used in the majority of tissue processing schedules? We use alcoholic formalin on our VIP. -----Original Message----- From: eileen dusek [mailto:eileen_dusek@yahoo.com] Sent: 04 November 2003 21:39 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] couple of questions Hi Everyone, I have a few questions, 1. Is anyone using a "home" type vacuum for the cryostat. Of course the bags would go in the medical waste. 2. Is Alcoholic formalin OK to put on the VIP tissue processor, there is no acetic acid in the solution. I appreciate all your help. Eileen signature _____ Do you Yahoo!? Protect your identity with Yahoo! Mail AddressGuard ************************************************************************ This e-mail is confidential and privileged. If you are not the intended recipient, please accept our apologies. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents, to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. North Bristol NHS Trust does not guarantee that this material is free from viruses or any other defects, although due care has been taken to minimise the risk. No contracts may be concluded on behalf of North Bristol NHS Trust by means of email communications. Thank you for your cooperation. ************************************************************************ ------_=_NextPart_001_01C3A3BD.C0D3779A Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable
Answer=20 to question 2: Why would it not be OK to use alcoholic formalin since = both, as=20 separate solutions, are used in the majority of tissue processing = schedules? We=20 use alcoholic formalin on our VIP.
-----Original Message-----
From: eileen dusek=20 [mailto:eileen_dusek@yahoo.com]
Sent: 04 November 2003=20 21:39
To: = histonet@lists.utsouthwestern.edu
Subject:=20 [Histonet] couple of questions

Hi Everyone,
I have a few questions,
1. Is anyone using a "home" type vacuum for the cryostat. Of = course the=20 bags would go in the medical waste.
2. Is Alcoholic formalin OK to put on the VIP tissue processor, =3D there is=20 no acetic acid in the solution.
 
I appreciate all your help.
 
Eileen


signature


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Protect=20 your identity with Yahoo! Mail = AddressGuard


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This e-mail is confidential and =3D privileged. If you are not the intended recipient, please accept our = apologies. Please do not disclose, copy or distribute information in =3D this e-mail or take any action in reliance on its contents, to do so is = strictly prohibited and may be unlawful. Please inform us that this =3D message has gone astray before deleting it. North Bristol NHS Trust = does not guarantee that this material is free from viruses or any other = defects, although due care has been taken to minimise the risk. No =3D contracts may be concluded on behalf of North Bristol NHS Trust by = means of email communications.

Thank you for your cooperation. =3D

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------_=_NextPart_001_01C3A3BD.C0D3779A-- --__--__-- Message: 14 From: "Thom Jensen" To: histonet@lists.utsouthwestern.edu Date: Wed, 05 Nov 2003 17:26:35 +0000 Subject: [Histonet] Re: Histonet digest, Vol 1 #116 - 24 msgs
In regard to Instrumedics comments, The reciepent block is the blank paraffin block used as the array block for inserting your cores into.
 
I do not recommend using the Tape-Transfer System for paraffin sectioning.  I know of no one who has had success using it for paraffin.  The sections are compressed and often unreadable.
 
Thom Jensen
 
For more information on Tissue Microarray instruction visit:  www.arrayworkshop.com
 
 
 


Is your computer infected with a virus? Find out with a FREE computer virus scan from McAfee. Take the FreeScan now! --__--__-- Message: 15 Date: Wed, 5 Nov 2003 12:46:12 -0500 (EST) From: Ramesh Subrahmanyam To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Distinguishing monocytes from granulocytes Hello Histonetters, I am trying to distinguish immature monocytes from immature granulocytes in Balb/c mice. Can anyone suggest antibodies for FACS or immunohistochemistry or RT-PCR markers that would help in this? Suggestions on other methods would be welcome too. Thanks in advance, Ramesh Brandeis University Massachusetts --__--__-- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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