[Histonet] URGENT! Questions regarding snap freezing in liquid nitrogen with isopentene
I am an Honours student, currently finishing up my Honours thesis.
I am wondering if anyone could give me some REFERENCES on snap freezing mouse
tissues using liquid nitrogen and ISOPENTENE. I have used liquid nitrogen ONLY
for snap freezing my mouse tissues in my project, but not with isopentene. And
i have been having cryostat sectioned mouse tissues that have a lot of tissue
degeneration, as well as artefacts after fixing in 2% para & 0.5% Glu, and i
got the idea from someone that this maybe due to the fact that i did not use
isopentene in the snap freezing process.
So what i want is to discuss this in my thesis. So it would be great if
someone could give me any scientific references/comments/advice on this method
whereby isopentene is used in the snap freezing process, and why using this is
I have been doing some internet searching myself, but any advice or help from
all histo experts out there would be greatly appreciated.
Hong Yuan Khor
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