[Histonet] Trichromes (was Re:What is Histology?)

From:John Kiernan

The last 2 paragraphs of this email are not
about trichrome staining.

Are you (SAULSAULX@aol.com) looking for a 
review of postulated mechanisms of differential 
staining in the various trichrome methods, or 
for 10 pages of practical instructions, or 
for comparisons of different trichrome methods? 
If you let us know what you need you (and
other Histonetters) will probably get several 
reading lists! 

The staining mechanisms are controversial 
because there has been too much speculation
and not enough research. In this field you'll
need to read 3 or 4 review articles to try
to form a balanced view of your own. The 
mechanisms for trichromes (methods using 3
anionic dyes and PTA or PMA) need to be
considered alongside methods using 2
anionic dyes in one solution (notably
Van Gieson's stain and its less often
used congeners). 

Is your name really SAULSAULX? It's much
more satisfying to reply to a name at a 
real location such as, for example,
Abey C. Defghi at the Royal Spithouse Infirmary 
in Badchester, Rayneshire, England. 
Histonet is a friendly community of over 
1000 (I think; Herb or Linda might correct
the number), and most of us indicate who we are
when we ask or answer questions.

On this note (and nothing to do with trichrome
stains), I'd like to say how happy I was to
see many Histonetters face to face at the 
recent NSH meeting in Louisville. I wish I
could have talked with more of you, but it's
easy to miss people in a big crowd. Also, the
names on the lapel badges were printed too
small. Such badges should be readable at a
distance. Though not a shy person, I did not
want to stare closely at the name label on
every comely bosom. In these litiginous times
everyone must avoid being greatly misunderstood. 
(NSH label makers please take note! Not many 
people have telescopic vision. Our instrument 
is the microscope.)
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
SAULSAULX@aol.com wrote:
> I need a ten page research paper on the trichrome stain can anyone help me/
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