I will start a work with lipids stain and I have some basic questions. In
the protocol for Sudan Black and Oil Red O say:
Solution of Sudan Black 0.4% in ethanol 70%: dissolve and place at
37ēC for 12 hours, wait to get cold and filter (solution stable for 15
And the same for the solution of oil red O, but in this case I don´t have
any indication of the validity.
And my questions are: Is necessary that the solutions stay at 37ēC for 12
hours "exactly" or is possible reduce this time (or extend?) and wath are your
opinion about the validity and conditions of storage?
Tanks in advance