[Histonet] Re: answers on folded sections

From:Nada Ben Abdallah


Dear colleagues,
thank you for your replies. 
to answer your question hernan, this perfusion protocol is used for both
Timm and neurogenesis. that's why I used it with my mice. As for the drying
of the slides, I already left the slides ON at room temperature but as you
suggested stephanie I will leave them longer and maybe try at 40C.
Hope this will work.

Sincerely,
nada


At 08:49 18.11.03 -0800, slieblich@shaw.ca wrote:
>I have also done 40um sections and nissl.  My suggestion is to let them
dry at least over night (up to a couple of days) before staining.  Further,
you can put them in a humidifier (or water bath, but just above the water
surface)for 10-20 mins and let them really stick on.  That's all I can
think of, or maybe use subbed slides....
>Hope this helps!
>
>Stephanie Lieblich
>University of British Columbia
>
>----- Original Message -----
>From: Nada Ben Abdallah 
>Date: Tuesday, November 18, 2003 2:27 am
>Subject: [Histonet] (no subject)
>
>> 
>> 
>> Dear all,
>> I am a freshly starting PhD student and my project concerns mainly 
>> dealingwith mouse brain frozen sections. My first work was just to 
>> practicehistology was to cut 40 microns coronal sections and nissl 
>> stain them.
>> Everything worked fine except that the sections started to fold when
>> stained (although I mounted them on a gelatine coated slides and used
>> gelatine to fix them more on the slides).
>> PS: the mouse from which the brain was taken was perfused with 
>> this procedure:
>> -PBS
>> -sulfid
>> -parafolmaldehyde+picric acid
>> and then the brain was placed in 30%sucrose for long time until used.
>> 
>> I appreciate it if you have any suggestions before I proceed with 
>> my work.
>> 
>> Best wishes,
>> nada
>> 
>> 
>> 
>> 
>> 
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>
>


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