[Histonet] Re: Histonet digest, Vol 1 #133 - 27 msgs

From:Cindy DuBois

There are 3 histolabs around my area and all of us start no later than 4
a.m.



on 11/17/03 10:00 AM, histonet-request@lists.utsouthwestern.edu at
histonet-request@lists.utsouthwestern.edu wrote:

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> 
> Today's Topics:
> 
> 1. what is histology (Michael F.)
> 2. re what is histology? (Carl)
> 3. RE: Perfusion pump time (Charles W. Scouten, Ph.D.)
> 4. RE: e-cadherin antibody (Scott Mordue)
> 5. Re: e-cadherin antibody (SAULSAULX@aol.com)
> 6. Re: Decreased antigenicity with alcohol-formalin fixation (anita dudley)
> 7. Re: e-cadherin antibody (SAULSAULX@aol.com)
> 8. Trichromes (was Re:What is Histology?) (John Kiernan)
> 9. cytokeratin (Lombaert)
> 10. Re: Exhaust Block (louise renton)
> 11. RE: thick frozen sections (Alan Bright)
> 12. Re: Trichromes (was Re:What is Histology?) (SAULSAULX@aol.com)
> 13. Re: Trichromes (was Re:What is Histology?) (rfail@toolkitmail.com)
> 14. Fwd: Re: [Histonet] Trichromes (was Re:What is Histology?) (Ian
> Montgomery)
> 15. Re: cytokeratin (Dennis Najarian)
> 16. Re: Trichromes (was Re:What is Histology?) (Bryan Hewlett)
> 17. Hansson's Carbnic Anhydrase. (leanneharris@eircom.net)
> 18. What is histology? (Dawson, Glen)
> 19. RE: Anti-beta-galactosidase antibodies (Favara, Cynthia (NIH/NIAID))
> 20. RE: Hansson's Carbnic Anhydrase. (Houston, Ronnie)
> 21. polymerization failure _ Technovit 9100New (Tom Schaer)
> 22. Re: Re: [Histonet] Trichromes (Hernan Aldana Marcos)
> 23. Trichromes and "ten-page double-spaced paper" (Alex Knisely)
> 24. trichrome paper (Mary Lou)
> 25. Re: Trichromes (was Re:What is Histology?) (Gayle Callis)
> 26. Re: CSAII kit (Dana Settembre)
> 27. RE: re what is histology? (Chung, Luong)
> 
> --__--__--
> 
> Message: 1
> To: histonet@lists.utsouthwestern.edu
> From: "Michael F." 
> Organization: Waikiki Coffee Inc.
> Date: Sun, 16 Nov 2003 09:31:58 -1000
> Subject: [Histonet] what is histology
> 
> as an aside, could you folks tell what percent would you say of HT/HTL
> start work before 5am?
> 
> would you say that an HT/HTL would be more likely to have opportunities to
> work in research than a MT/MTL?
> 
> I'm considering one or the other schools. Hope you don't mind my off topic
> posting. Please email me directly if its more appropriate.
> 
> cheers,
> 
> M.
> 
> ...back to lurking
> 
> 
> --__--__--
> 
> Message: 2
> From: "Carl" 
> To: 
> Date: Sun, 16 Nov 2003 19:52:55 -0000
> Subject: [Histonet] re what is histology?
> 
> After a friend asked what I am/did, I expained about Histology, staining,
> dyes etc. I subsequently overheard him saying to another person that I was
> an artist! To him dyes/stains= paints...( hadn't listened to a word....or my
> explanation was inadequate  lol)
> I suppose it's true; a picture paints a thousand words. Perhaps that's why,
> for example, the pathologist uses many words to describe what's seen in a
> section  ;-)
> 
> 
> 
> --__--__--
> 
> Message: 3
> Date: Sun, 16 Nov 2003 17:20:17 -0600
> From: "Charles W. Scouten, Ph.D." 
> To: "Davis, Gareth" ,
> "Histonet" 
> Subject: RE: [Histonet] Perfusion pump time
> 
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> I have responded separately with an attached  7 page discussion of
> perfusion, and a link to our Perfusion One product.  Don't use flow
> rate, pressure is what the cardiovascular system controls,  use 5%
> sucrose prewash, no saline, and switch to fixative when the animal
> stops.  Haparin is not needed, all blood can be cleared without it with
> sufficient pressure.
> 
> =20
> 
> If any body else would like to see these materials, I will send them.
> 
> =20
> 
> Charles W.  Scouten, Ph.D.=20
> myNeuroLab.com=20
> 5918 Evergreen Blvd.=20
> St. Louis, MO 63134=20
> Ph: 314 522 0300 =20
> FAX  314 522 0377=20
> cwscouten@myneurolab.com  =20
> www.myneurolab.com=20
> 
> =20
> 
> -----Original Message-----
> From: Davis, Gareth [mailto:gareth.davis@Vanderbilt.Edu]=20
> Sent: Saturday, November 15, 2003 3:29 PM
> To: Histonet
> Subject: [Histonet] Perfusion pump time
> 
> =20
> 
> Hello Histonetters,
> 
> Recently (within the last couple months) I saw someone post perfusion
> times for pumps based on the animal being perfused.  I tried to find the
> posting on the Histosearch, but unsuccessful.  We are perfusing rats for
> the olfactory tissue, but would like ideals on the what others have
> done.  Any input would be appreciated, including if heparin is needed in
> saline, the correct perfusion pump time, and if recirculation is
> necessary.
> 
> thanks,
> 
> Gareth
> 
> =20
> 
> Ms. Gareth B. Davis
> 
> Research Assistant II
> 
> =20
> 
> =20
> 
> 
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> >

Roman"> style=3D'font-size:12.0pt;color:navy'>I have responded separately with = > an attached >  7 page discussion of perfusion, and a link to our Perfusion One > product.  Don’t use flow rate, pressure is what the = > cardiovascular > system controls,  use 5% sucrose prewash, no saline, and switch to = > fixative > when the animal stops.  Haparin is not needed, all blood can be = > cleared without > it with sufficient pressure.

> >

Roman"> style=3D'font-size:12.0pt;color:navy'> 

> >

Roman"> style=3D'font-size:12.0pt;color:navy'>If any body else would like to see = > these > materials, I will send them.

> >

Roman"> style=3D'font-size:12.0pt;color:navy'> 

> >
> >

style=3D'font-size:12.0pt; > font-family:"Comic Sans MS";color:navy'>Charles W.  Scouten, = > Ph.D. color=3Dnavy>
>
style=3D'color:#339966;font-weight: > bold'>myNeuroLab.com style=3D'color:navy'> >
>
Style"> style=3D'font-size:10.0pt;font-family:"Bookman Old = > Style";color:navy'>5918 > Evergreen Blvd. style=3D'color:navy'>
>
Style"> style=3D'font-size:10.0pt;font-family:"Bookman Old = > Style";color:navy'>St. Louis, > MO 63134 = >
>
Style"> style=3D'font-size:10.0pt;font-family:"Bookman Old = > Style";color:navy'>Ph: 314 522 > 0300  style=3D'color:navy'>
>
Style"> style=3D'font-size:10.0pt;font-family:"Bookman Old = > Style";color:navy'>FAX  > 314 522 0377 = >
> cwscouten@myneurolab.com = >
> www.myneurolab.com

> >
> >

Roman"> style=3D'font-size:12.0pt;color:navy'> 

> >

face=3DTahoma> style=3D'font-size:10.0pt;font-family:Tahoma'>-----Original = > Message-----
> From: Davis, Gareth > [mailto:gareth.davis@Vanderbilt.Edu]
> Sent: Saturday, November = > 15, 2003 > 3:29 PM
> To: Histonet
> Subject: [Histonet] = > Perfusion pump > time

> >

face=3D"Times New Roman"> style=3D'font-size:12.0pt'> 

> >
> >

face=3D"Times New Roman"> style=3D'font-size:12.0pt'>Hello Histonetters,

> >
> >
> >

face=3D"Times New Roman"> style=3D'font-size:12.0pt'>Recently (within the last couple months) I = > saw someone > post perfusion times for pumps based on the animal being = > perfused.  I > tried to find the posting on the Histosearch, but unsuccessful.  We = > are > perfusing rats for the olfactory tissue, but would like ideals on the = > what > others have done.  Any input would be appreciated, including if = > heparin is > needed in saline, the correct perfusion pump time, and if > recirculation is necessary.

> >
> >
> >

face=3D"Times New Roman"> style=3D'font-size:12.0pt'>thanks,

> >
> >
> >

face=3D"Times New Roman"> style=3D'font-size:12.0pt'>Gareth

> >
> >
> >

face=3D"Times New Roman"> style=3D'font-size:12.0pt'> 

> >
> >
> >

color=3Dpurple > face=3DSystem> style=3D'font-size:10.0pt;font-family:System;color:purple'>Ms. > Gareth B. Davis

> >
> >
> >

size=3D2 > color=3Dpurple face=3DSystem> style=3D'font-size:10.0pt;font-family:System; > color:purple'>Research Assistant II

> >
> >
> >

face=3D"Times New Roman"> style=3D'font-size:12.0pt'> 

> >
> >
> >

face=3D"Times New Roman"> style=3D'font-size:12.0pt'> 

> >
> >
> > > > > =00 > ------_=_NextPart_001_01C3AC98.32688D72-- > > > --__--__-- > > Message: 4 > Date: Sun, 16 Nov 2003 18:03:16 -0800 (PST) > From: Scott Mordue > To: alexander.nader@wgkk.sozvers.at > Cc: Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] e-cadherin antibody > > We are using monoclonal E-Cadherin in breast cancer > tissues, clone: 4A2C7, from Zymed. > > Scott > CSU > > > From: "Nader, Alexander" > > To: "'histonet-admin@lists.utsouthwestern.edu'" > > Cc: "'Histonet@lists.utsouthwestern.edu'" > > Date: Wed, 12 Nov 2003 08:07:01 +0100 > Subject: RE: [Histonet] e-cadherin antibody > >> Which clone are people using for e-caherin IHC on > FFPE tissues? >> Novocastra E-Cadherin clone 36B5 (IgG1) 1:50. > > Alexander Nader > Inst. f. Pathology, Hanusch-Hospital > Vienna, Austria > > __________________________________ > Do you Yahoo!? > Protect your identity with Yahoo! Mail AddressGuard > http://antispam.yahoo.com/whatsnewfree > > > --__--__-- > > Message: 5 > From: SAULSAULX@aol.com > Date: Sun, 16 Nov 2003 21:12:34 EST > To: i_stain@yahoo.com, alexander.nader@wgkk.sozvers.at > CC: Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] e-cadherin antibody > > > --part1_1a5.1c1895a5.2ce98892_boundary > Content-Type: text/plain; charset="US-ASCII" > Content-Transfer-Encoding: 7bit > > Histotechies......! I need help.where can i get a ten page double space > research paper on the following two stains: > > 1.Alcian Blue > > 2.Trichrome stain > > > thanks > > --part1_1a5.1c1895a5.2ce98892_boundary > Content-Type: text/html; charset="US-ASCII" > Content-Transfer-Encoding: quoted-printable > > =3D"Arial" LANG=3D"0">Histotechies......! I need help.where can i > get=20= > a ten page double space research paper on the following two stains:
>
> 1.Alcian Blue
>
> 2.Trichrome stain
>
>
> thanks
> > --part1_1a5.1c1895a5.2ce98892_boundary-- > > > --__--__-- > > Message: 6 > From: "anita dudley" > To: GoodwinD@pahosp.com, HistoNet@Pathology.swmed.edu > Date: Mon, 17 Nov 2003 02:13:03 +0000 > Subject: Re: [Histonet] Decreased antigenicity with alcohol-formalin fixation > > diana, I use penfix on my processor and we have good luck with our ers and > prs, we also use it sometime to fix breast tissue before going on the > processor. haven't had any problems with it. hope this helps. > anita dudley > providence hosp > mobile alabama > > >> From: "Goodwin, Diana" >> To: "Histonet (E-mail)" >> Subject: [Histonet] Decreased antigenicity with alcohol-formalin fixation >> Date: Fri, 14 Nov 2003 11:39:21 -0500 >> >> >>> Greetings, Histonetters. >>> >>> One of my pathologists has expressed concern that antigenicity for >> certain >>> antibodies, specifically ER and PR, in breast tissue that is processed >>> using alcohol-formalin will be decreased. Can anyone cite a reference >> in >>> the literature that confirms or addresses this concern? Has anyone had >>> this experience, or does anyone know of any reason that using >>> alcohol-formalin (specifically Pen-Fix) would adversely affect IHC >>> staining? >>> >>> Thanks to all of you, >>> >>> Diana Goodwin >>> Supervisor, Anatomic Pathology >>> Preston 6 >>> 215-829-6532 >>> e-mail: goodwind@pahosp.com >>> > > _________________________________________________________________ > Frustrated with dial-up? Get high-speed for as low as $26.95. > https://broadband.msn.com (Prices may vary by service area.) > > > > --__--__-- > > Message: 7 > From: SAULSAULX@aol.com > Date: Sun, 16 Nov 2003 21:15:04 EST > To: SAULSAULX@aol.com, i_stain@yahoo.com, alexander.nader@wgkk.sozvers.at > CC: Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] e-cadherin antibody > > > --part1_28.40452994.2ce98928_boundary > Content-Type: text/plain; charset="US-ASCII" > Content-Transfer-Encoding: 7bit > > In a message dated 11/16/2003 9:14:01 PM Eastern Standard Time, > SAULSAULX@aol.com writes: > >> Histotechies......! I need help.where can i get a ten page double space >> research paper on the following two stains: >> >> 1.Alcian Blue >> >> 2.Trichrome stain >> >> >> thanks > > Histotechies......! I need help.where can i get a ten page double space > research paper on the following two stains: > > 1.Alcian Blue > > 2.Trichrome stain > > > thanks > > --part1_28.40452994.2ce98928_boundary > Content-Type: text/html; charset="US-ASCII" > Content-Transfer-Encoding: quoted-printable > > =3D"Arial" LANG=3D"0">In a message dated 11/16/2003 9:14:01 PM Eastern Stand= > ard Time, SAULSAULX@aol.com writes:
>
>
: 5px; MARGIN-RIGHT: 0px; PADDING-LEFT: 5px">Histotechies......! I nee= > d help.where can i get a ten page double space research paper on the followi= > ng two stains:
>
> 1.Alcian Blue
>
> 2.Trichrome stain
>
>
> thanks
ZE=3D3 FAMILY=3D"SANSSERIF" FACE=3D"arial" LANG=3D"0">
= >
>
FAMILY=3D"SANSSERIF" FACE=3D"Arial" LANG=3D"0">
> Histotechies......! I need help.where can i get a ten page double spac= > e research paper on the following two stains:
>
> 1.Alcian Blue
>
> 2.Trichrome stain
>
>
> thanks
ZE=3D3 FAMILY=3D"SANSSERIF" FACE=3D"arial" LANG=3D"0">
>
> --part1_28.40452994.2ce98928_boundary-- > > > --__--__-- > > Message: 8 > Date: Mon, 17 Nov 2003 00:47:47 -0500 > From: John Kiernan > Reply-To: jkiernan@uwo.ca > To: SAULSAULX@aol.com > CC: Jackie.O'Connor@abbott.com, tigersnake@ecybermind.net, > histonet@lists.utsouthwestern.edu > Subject: [Histonet] Trichromes (was Re:What is Histology?) > > The last 2 paragraphs of this email are not > about trichrome staining. > > Are you (SAULSAULX@aol.com) looking for a > review of postulated mechanisms of differential > staining in the various trichrome methods, or > for 10 pages of practical instructions, or > for comparisons of different trichrome methods? > If you let us know what you need you (and > other Histonetters) will probably get several > reading lists! > > The staining mechanisms are controversial > because there has been too much speculation > and not enough research. In this field you'll > need to read 3 or 4 review articles to try > to form a balanced view of your own. The > mechanisms for trichromes (methods using 3 > anionic dyes and PTA or PMA) need to be > considered alongside methods using 2 > anionic dyes in one solution (notably > Van Gieson's stain and its less often > used congeners). > > Is your name really SAULSAULX? It's much > more satisfying to reply to a name at a > real location such as, for example, > Abey C. Defghi at the Royal Spithouse Infirmary > in Badchester, Rayneshire, England. > Histonet is a friendly community of over > 1000 (I think; Herb or Linda might correct > the number), and most of us indicate who we are > when we ask or answer questions. > > On this note (and nothing to do with trichrome > stains), I'd like to say how happy I was to > see many Histonetters face to face at the > recent NSH meeting in Louisville. I wish I > could have talked with more of you, but it's > easy to miss people in a big crowd. Also, the > names on the lapel badges were printed too > small. Such badges should be readable at a > distance. Though not a shy person, I did not > want to stare closely at the name label on > every comely bosom. In these litiginous times > everyone must avoid being greatly misunderstood. > (NSH label makers please take note! Not many > people have telescopic vision. Our instrument > is the microscope.) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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