[Histonet] RE: dystrophin

From:"C.M. van der Loos"

Dear Heather,
If you don't see anything after testing these dystrophin antibodies on 
FFPE tissues you may try the following (as last resort perhaps):
- Good old pepsin digestion (instead of HIER) using 0.25% pepsin 
(Sigma) in 10 mM HCl. 15 min at 37C. 
- A stronger detection system. Try for example the new CSA II kit from 
DakoCyto. A non-biotin based peroxidase staining kit that is very 
powerful. Test your antibodies in steps like: 1:50 - 200 - 1000 - 5000.
Good luck,

Chris van der Loos
Academical Medical Center
Dept. of Cardiovascular Pathology
Amsterdam - The Netherlands

----- Original Message ----- 
From  Mcleod  
Date  Tue, 04 Nov 2003 07:15:44 +0200 
To  "histonet@lists.utsouthwestern.edu" 
Subject  [Histonet] dystrophin 


Dear All

Would anybody be able to help with the above antibody? A researcher who
has never done any IHC before bought the following Novocastra Antobodies
(1) NCL-DYS1 (2) NCL-DYS2 and (3) NCL-DYS3.  These however are for
frozen sections and she needs to work with FFPE material.  A first
attempt on the ffpe sections gave no staining. Has anybody by any chance
ever had the need to use them?  ANY help will be greatly appreciated.
Heather McLeod

Histonet mailing list

<< Previous Message | Next Message >>