[Histonet] RE: dystrophin
Dear Heather,
If you don't see anything after testing these dystrophin antibodies on
FFPE tissues you may try the following (as last resort perhaps):
- Good old pepsin digestion (instead of HIER) using 0.25% pepsin
(Sigma) in 10 mM HCl. 15 min at 37C.
- A stronger detection system. Try for example the new CSA II kit from
DakoCyto. A non-biotin based peroxidase staining kit that is very
powerful. Test your antibodies in steps like: 1:50 - 200 - 1000 - 5000.
Good luck,
Chris van der Loos
Academical Medical Center
Dept. of Cardiovascular Pathology
Amsterdam - The Netherlands
----- Original Message -----
From Mcleod
Date Tue, 04 Nov 2003 07:15:44 +0200
To "histonet@lists.utsouthwestern.edu"
Subject [Histonet] dystrophin
------------------------------------------------------------------------
Dear All
Would anybody be able to help with the above antibody? A researcher who
has never done any IHC before bought the following Novocastra Antobodies
(1) NCL-DYS1 (2) NCL-DYS2 and (3) NCL-DYS3. These however are for
frozen sections and she needs to work with FFPE material. A first
attempt on the ffpe sections gave no staining. Has anybody by any chance
ever had the need to use them? ANY help will be greatly appreciated.
Thankyou
Heather McLeod
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