[Histonet] RE: Biotin block after primary?
As as long as you haven't applied the biotinylated secondary antibody
and third step streptavidin-reagent, you don't have any problem with
biotin blocking after the primary antibody.
The trick of usual 2-step biotin-blocking kits is to bind (strept)
avidin to the intrinsic biotin in the tissue section first and then to
saturate the left-over binding sites at the (strept)avidin molecule
with free d-biotin.
Chris van der Loos
Dept. of Cardiovascular Pathology
Academical Medical Center
Amsterdam - The Netherlands
----- Original Message -----
From "brade, brade upland, upland"
Date Wed, 19 Nov 2003 20:02:23 -0800 (PST)
Subject [Histonet] Biotin block after primary?
I accidently added my primary antibody before avidin/biotin blocking.
Is it possible to block after the primary but before a biontinylated
secondary? Thank you for help.
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