[Histonet] Perfusions of young mice
If anyone has any advice on perfusing very young mice I would be grateful
to hear it. We are trying to harvest the brains of mice at P9 (post-natal
day 9) and younger. I've been able to perfuse adult mice with the standard
method of injecting preperfusion and paraformaldehyde solutions into the
left ventricle, with a cut in the right atrium pre-injection.
My biggest problem, as I recently tried perfusing a P9 mouse, is that the
heart is so small that I have yet to successfully place the needle in the
left ventricle and inject without going through and getting little to no
perfusion. That and accessing the right atrium to cut it and allow outflow.
The heart is quite small.
Randa M. Perkins
Florida State University
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