[Histonet] Perfusions of young mice

From:"Randa Perkins"

To all:
  If anyone has any advice on perfusing very young mice I would be grateful 
to hear it.  We are trying to harvest the brains of mice at P9 (post-natal 
day 9) and younger.  I've been able to perfuse adult mice with the standard 
method of injecting preperfusion and paraformaldehyde solutions into the 
left ventricle, with a cut in the right atrium pre-injection.
  My biggest problem, as I recently tried perfusing a P9 mouse, is that the 
heart is so small that I have yet to successfully place the needle in the 
left ventricle and inject without going through and getting little to no 
perfusion.  That and accessing the right atrium to cut it and allow outflow. 
  The heart is quite small.
  Any suggestions?

  Randa Perkins

Randa M. Perkins
Research Assistant
Florida State University
Tallahassee, FL

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