[Histonet] CD 4/CD 8 staining of frozen stomach sections of mice
Hello everyone,
I am trying (for a long time allready) to produce a
good CD4/CD8 staining of frozen stomach tissue (mice). We use the Dako rat
anti-mouse CD4 antibody but always encounter a large amound of background
after staining (it seems like there is a lot of cross reactivity with the
glandular epithelium). Can anyone help me to solve this problem ?
Thanx !
Manuelle De Bock, dierenarts (DVM)
Laboratory of
Veterinary Pathology
Department of Pathology, Bacteriology and Avian
Diseases
Faculty of Veterinary Medicine
Ghent University
Salisburylaan
133
B9820 Merelbeke - Belgium
Tel 0032(0)9 264 7745
Fax 0032(0)9 264
7789
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