[Histonet] Bielschowsky's stain
Does anyone have good result with BIELSCHOWSK'S
I am using the technique from Bancroft as follows
on brain tissue of dogs:
1. Deparaffinize and hydrate to distilled water.
Wash well in distilled water.
2. Place sections in 50 ml of 20% silver nitrate in
the dark at 37 C. Keep this silver solution for
3. Wash well in distilled water.
4. Add strong ammonia to 50 ml of silver nitrate
(from stage 2) until the initial precipitate disappears.
Leave section in this solution for 10 minutes at 37
I would to understand step 4. Should I add
I added concentrated ammonia 1 to 2 drop at a time.
Immediately I got precipitate that did not disappear.
I repeated this step with dilute ammonia getting
the same result. I filtered the soluton and continued the
staining and obtained no color on my
I guess I have a problem with step 4. How
much volume of ammonia should be added at what concentration. Is there a
critical point in this staining.
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