Fwd: [Histonet] cytokeratin

From:Ian Montgomery

        Used Cytokeratin 8 from Dako after Trysin on mouse tissue followed by Dako Ark. Beautiful results.

From: "Lombaert" <i.lombaert@med.rug.nl>
Organization: faculty of medical sciences (RuG)
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Date: Fri, 7 Nov 2003 14:58:33 +0100
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Subject: [Histonet] cytokeratin
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Dear Histonetters,

Has anyone tried to stain cytokeratins (7, 8.12, 14 and 18 from
Sigma) on mouse/murine salivary gland tissues? I already tried 10
mM sodium citrate pH 6.0 for 30 min in the microwave,

as well as 0.1M TrisHCl pH 9.5,
no target retrieval at all,
proteinase K for 5 min (20 mg/ml)

but no results. I only could detect something with the enzym
proteinase K, although my tissue was disrupted badly.
My sections are paraffine imbedded and 4% formaldehyde fixed.

Kind regards,

Ir. Isabelle Lombaert, MSc
PhD student
University of Groningen
Faculty of Medical Sciences
Antonius Deusinglaan 1
Building 3215, 5th floor, Room 553
9713 AV Groningen
The Netherlands
e-mail: I.Lombaert@med.rug.nl
Tel.: +31 (0)50 363 29 15

Histonet mailing list

Dr. Ian Montgomery,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07625 702883
e-mail: ian.montgomery@bio.gla.ac.uk

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