From: "Lombaert" <i.lombaert@med.rug.nl>
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Date: Fri, 7 Nov 2003 14:58:33 +0100
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Subject: [Histonet] cytokeratin
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Dear Histonetters,

Has anyone tried to stain cytokeratins (7, 8.12, 14 and 18 from
Sigma) on mouse/murine salivary gland tissues? I already tried 10
mM sodium citrate pH 6.0 for 30 min in the microwave,

as well as 0.1M TrisHCl pH 9.5,
no target retrieval at all,
proteinase K for 5 min (20 mg/ml)

but no results. I only could detect something with the enzym
proteinase K, although my tissue was disrupted badly.
My sections are paraffine imbedded and 4% formaldehyde fixed.


Kind regards,
Isabelle


-----------------------
Ir. Isabelle Lombaert, MSc
PhD student
University of Groningen
Faculty of Medical Sciences
Antonius Deusinglaan 1
Building 3215, 5th floor, Room 553
9713 AV Groningen
The Netherlands
e-mail: I.Lombaert@med.rug.nl
Tel.: +31 (0)50 363 29 15

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