Re: perl staining

From:Nancy E Weber

Hi Neelima,
Here's one, it's quite easy.

Fixative:  10% neutral buffered formalin.
Technique:  Cut paraffin sections at 3 to 5 microns.
Quality Control:  Section containing ferric iron.

Staining Procedure:
1. Deparaffinize and hydrate sections to distilled water.
2. Place slides in potassium ferrocyanide-hydrochloric acid for 10 min.
3.  Rinse well in distilled water.
4.  Counterstain with nuclear fast red for 5 min.
5.  Rinse in distilled water.
6. Dehydrate, clear , and mount.

Results:
    Ferric Iron..............................bright blue
    Nuclei.............................................red
    Cytoplasm...............................light pink

Solutions:
    2% Potassium Ferocyanide
        Potassium Ferrocyanide...........................2.0 gm
       Distilled water........................................100.0 ml

    2% Hydrochloric Acid
       Hydrochloric Acid.....................................2.0 ml
       Distilled Water.......................................98.0 ml

    Potassium Ferrocyanide-Hydrochloric Acid Solution
       2% Potassium Ferrocyanide......................25.0 ml
       2% Hydrochloric Acid..............................25.0 ml

    Nuclear Fast Red
       Aluminum Sulfate........................................5.0 gm
       Distilled water..........................................100.0 ml
       Nuclear Fast Red........................................0.1 gm
       Dissolve aluminum sulfate in distilled water, then add nuclear fast
red and dissolve with aid of heat.  Cool, filter, and add a grain of
       thymol as a perservative.

Reference:
      Bancroft, J. D., Stevens, A. 1982  "Theory and Practice of
Histological Techniques". (2nd ed.) (p 258).



Nancy Weber
Research Associate
Veterinary Clinical Sciences
Ohio State University
----- Original Message -----
From: "Neelima Shah" 
To: 
Sent: Thursday, November 21, 2002 5:45 PM
Subject: perl staining


> Hi,
> I looking for the protocol for perl staining. Thanks in advance.
>
>
>
>




<< Previous Message | Next Message >>