Re: brain stains

From:Geoff McAuliffe

Any Nissl-type stain will do. Cresyl violet, thionine, toluidine blue all work
well on frozen sections. Tol blue washes out with alcohol so use 2-3 changes of
acetone for dehydration. Simple, fast and, at least with tol blue, myelin is a
bit metachromatic.


"Martin, Ronald" wrote:

> Fellow techs,
> I have a PI who wants to look at Purkinje cells in mouse brains. I am use to
> doing silver stains on ffpe tissue however she has some tissue frozen in OCT
> via liquid nitrogen. Any suggestions other than having her harvest some
> tissue for ffpe ?
> Thanks,
> Ron Martin

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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