Re: Special Stains for Alzheimer's Disease
Re: Special Stains for Alzheimer's
Disease
Dear Ryan,
These are all immunhistochemical stains and
these three antibodies (b-amyloid,Tau,A-synuclein) are easily obtained
commercially, and will clearly demonstrate these features of
Alzheiner's Disease and Lewy body disease.
Belchowski silver stain will
show plaques and tangles too but not as clearly as the immuno
stains.
If you are not set up for immuno, any lab that regularly does
human autopsy material, should have working protocols for these
stains. It will probably be easier (cheaper and less time consuming)
to send the slides away if this is a one off case.
Good Luck, Cath
Please help
me with this inquiry.
A patient from the hospital has a pre-op of Alzheimer's Disease.
We have a portion of cerebral tissue to work with. My
pathologist requires the following stains to be done:
1. A stain for beta amyloid to characterize neuritic
plaques.
2. A stain for tau paired helical filaments to characterize
neurofibrillary tangles.
3. A stain for alpha synuclein to look for Lewy
bodies.
Which stains
are we required to do to represent these elements? The only stain I am
familiar with is the Belchowski (spelling check) stain.
All suggestions are welcomed and greatly appreciated.
Ryan
Lead Tech
Allan Memorial
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Catherine Gorrie
School of Medical Sciences,
University of New South Wales
Sydney, N.S.W. 2052
Phone: 61-2-9385 2462
Fax : 61-2-9313 6252
e-mail: c.gorrie@unsw.edu.au
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