Katri

I've had similar trouble with the signal using anti-EGFR from several
companies.  Try using "TrueBlue" from KPL.
It's far far more sensitive than DAB or other like reagents and will make
you happy.

Truly yours,

John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL  36849


On Fri, 22 Nov 2002, Katri Tuomala wrote:

> Hi all!
> Has anyone had experience with the antibody to epidermal growth factor receptor, Dako, clone H11. on FFPE human tissue?
> I've been trying to get it to work on normal tissues, that manufacturer claims it stains and only one that shows any staining is placenta. I've tried several retrieval techniques to no avail.
> Has anyone got this particular antibody to work or do I need to look for another antibody? Please help!
> Katri
>
> Katri Tuomala
> Department of Pathology
> St.Joseph's Hospital
> Hamilton, Ontario, Canada
>
>