Re: Brain Bubbles (isopropanol) follow up
Boneslides@aol.com wrote:
> Understanding that I have absolutely NO EXPERIENCE with brain
> tissue, I am curious...is there a reason/benefit to processing
> in isopropanol rather than in ethanol or one of the blends
> designed for use in histology processing?
The short answer is - Choose the cheapest
anhydrous alcohol. Isopropanol is cheaper than
ethyl alcohol if you have to pay booze-based
taxes when you buy ethanol. If your lab can get
duty-free ethanol it may be cheaper than
isopropanol.
Poisoned or denatured alcohol (ethyl mixed with methyl
or propyl) is usually cheap but it may contain up to
5% water. It can be used as to make 70%, and 95% but
not "absolute" (water-free) alcohol.
What are "the blends designed for use in histology"
that you ask about? There's no magic in the business
of solvents.
--
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
kiernan@uwo.ca
http://publish.uwo.ca/~jkiernan/
<< Previous Message | Next Message >>