Re: Brain Bubbles (isopropanol) follow up

From:"J. A. Kiernan" wrote: 
> Understanding that I have absolutely NO EXPERIENCE with brain
> tissue, I am there a reason/benefit to processing
> in isopropanol rather than in ethanol or one of the blends
> designed for use in histology processing?

The short answer is - Choose the cheapest 
anhydrous alcohol. Isopropanol is cheaper than
ethyl alcohol if you have to pay booze-based 
taxes when you buy ethanol.  If your lab can get
duty-free ethanol it may be cheaper than 

Poisoned or denatured alcohol (ethyl mixed with methyl 
or propyl) is usually cheap but it may contain up to 
5% water. It can be used as to make 70%, and 95% but
not "absolute" (water-free) alcohol.     

What are "the blends designed for use in histology" 
that you ask about? There's no magic in the business
of solvents.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1

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