Re: Astrocytes-Special stain

From:Geoff McAuliffe

Cynthia Favara wrote:

> As previously mentioned GFAP works well in FFPE tissue I have used DAKO on
> humans, mouse, rat, mink, and ferret. If my small bubbly brain is up to
> snuff GFAP is not expressed on all astrocytes.

    Quite true, at least in mouse and rat. In at least some parts of the brain
astrocytes are present but are unreactive with GFAP or only weakly reactive.
Injury to that part of the brain will often cause marked increase in GFAP
reactivity in just 48-72 hours.

> It is strongly positive in
> most TSE diseases. I have been successful in increasing the sensitivity by
> employing various antigen retrieval techniques [ may not be a politically
> correct statement] but will not stain all astrocytes present.
>
> Oh yes it is Friday and I am having more that one moment!!!
>
> Hoppe you have a good weekend!
>
> c
> Cynthia Favara
> NIAID/NIH/RML/LPVD
> 903 South 4th Street
> Hamilton, MT 59840
> 406-363-9317
>
> -----Original Message-----
> From: Jo Dee Fish [mailto:jfish@gladstone.ucsf.edu]
> Sent: Thursday, November 21, 2002 10:26 AM
> To: HistoNet@pathology.swmed.edu
> Subject: Astrocytes-Special stain
>
> Hello Histonetters,
> Does anyone know of a special stain for astrocytes.  This would be
> for paraffin embedded brain.
> I know of Cajal's stain, but unfortunately it is for frozen sections.
> Thanks much,
> Jo Dee
> #003#Jo Dee Fish
> Research Technologist II
> Gladstone Institue of Cardiovascular Disease
>
> Telephone: (415) 695-3720
> Fax: (415) 285-5632
> E-mail: jfish@gladstone.ucsf.edu
>
> Mailing address:
> Gladstone Instutes
> P.O. Box 419100
> San Francisco, CA 94141-9100

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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