RE: silanizing slides

From:Chris van der Loos


Dear Rosalba Zumbo,
We have been using the following protocol for silanizing our slides:
Prepare a 2% solution of 3-aminopropyltriethoxy-silane (Sigma A3648) in 
acetone.
Soak slides for 20 seconds in organosilan/acetone solution.
Rinse briefly in two acetone baths.
Wash with distilled water and dry slides overnight at 37C.

Rosalba Zumbo wrote:
 >Date: 25 Nov 2002 08:32:36 -0600
 >From: Rosalba Zumbo 
 >Subject: Silanising slides
 >Hi All,
 >Does anyone please have a rliable method for silanising slides. Different
 >methods I have come across have varying results in regard to silver
 >staining.
 >Thanks
 >Rosalba Zumbo
 >Histology Manager
 >Department of Forensic Medicine
 >50 Parramatta Rd
 >Glebe NSW 2037
 >PH: 02 85847842
 >FAX: 02 95664573

Chris M. van der Loos
Academical Medical Center
Dept. of Cardiovascular Pathology
Amsterdam
The Netherlands




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