RE: cytology cell blocks

From:Tony Henwood

Deb,
I would recommend the plasma/thrombin method. You  use your normal histological fixation and processing technique. Makes any routine stains and IPXs easier to control. Not really tht antiquated. Still works well, like formalin!!
 
Another method is the warm agar technique. Beware the heat can inactivate some antigens for IPX
 

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html

-----Original Message-----
From: Histology, Metro Lab [mailto:histo@qcmetro.com]
Sent: Wednesday, 20 November 2002 3:20
To: histonet posting address
Subject: cytology cell blocks

I was asked by cytology to find out what other labs are doing to make cell blocks.  For years they have been putting absolute alcohol on the sediments and when they are bloody they are hard as rocks. Now they are trying the plasma/thrombin method, but it seems so antiquated.
Any suggestions?
Thanks,
Deb
Metro Lab
Davenport, Iowa


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