RE: collagen I antibody
Please have a look at the Calbiochem catalog or http://www.calbiochem.com.
There is one anti-collagen type I antibody for mouse tissue mentioned. We
at least had good results using their mouse anti-collagen I for human
tissue (clone I-8H5).
Chris van der Loos
Academical Medical Center
Dept. of Cardiovascular Pathology
>Date: 26 Nov 2002 11:19:19 -0600
>From: Harry Sweigard
>Subject: collagen I antibody
>Hello, I'm having difficulty finding a good source for collagen I
>antibody to use on mouse tissue. I'm hoping someone may be able to guide
>me in the right direction. Novocastra had one that worked great but they
>stopped making it. Biodesign has one that has very weak reactivity after
>high heat retrieval, which tends to do a number on the collagen leaving
>little to stain. The hybridoma bank has one but I called and she said it
>won't cross with mouse tissue. I've run out of ideas and was hoping
>someone could come along with a wonderful suggestion, or any suggestion
>for that matter.
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