RE: collagen I antibody

From:Chris van der Loos

Dear Harry,
Please have a look at the Calbiochem catalog or 
There is one anti-collagen type I antibody for mouse tissue mentioned. We 
at least had good results using their mouse anti-collagen I for human 
tissue (clone I-8H5).

Chris van der Loos
Academical Medical Center
Dept. of Cardiovascular Pathology
The Netherlands

Harry wrote:
 >Date: 26 Nov 2002 11:19:19 -0600
 >From: Harry Sweigard 
 >Subject: collagen I antibody
 >Hello, I'm having difficulty finding a good source for collagen I
 >antibody to use on mouse tissue. I'm hoping someone may be able to guide
 >me in the right direction. Novocastra had one that worked great but they
 >stopped making it. Biodesign has one that has very weak reactivity after
 >high heat retrieval, which tends to do a number on the collagen leaving
 >little to stain. The hybridoma bank has one but I called and she said it
 >won't cross with mouse tissue. I've run out of ideas and was hoping
 >someone could come along with a wonderful suggestion, or any suggestion
 >for that matter.
 >- -Harry

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