When you say she wants to look at Purkinje cells, what is she
looking 
     for? A simple Cresyl violet or thionin stain will show the purkinje

     cells in the cerebellum beautifully and may be sufficient for her 
     needs. Certainly can be performed on frozen sections
     
     Ronnie Houston
     Regional Histology Operations Manager
     Bon Secours HealthPartners Laboratories
     5801 Bremo Road
     Richmond, VA 23226


______________________________ Reply Separator
_________________________________
Subject: RE: brain stains
Author:  "Cynthia Favara"  at MSGHub
Date:    11/22/02 11:50 AM


I am not sure how the morphology will be but this might be worth a try. 
Place the OCT encased specimen in NBF and process. May be adequate for
what 
the PI wants to see.
     
c
Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317
     
     
-----Original Message-----
From: Martin, Ronald [mailto:Ronald.Martin@umassmed.edu] 
Sent: Thursday, November 21, 2002 10:11 AM
To: 'histonet@pathology.swmed.edu'
Subject: brain stains
     
     
Fellow techs,
I have a PI who wants to look at Purkinje cells in mouse brains. I am
use to 
doing silver stains on ffpe tissue however she has some tissue frozen in
OCT 
via liquid nitrogen. Any suggestions other than having her harvest some 
tissue for ffpe ?
Thanks,
Ron Martin