RE: RNA

From:Tony Henwood

RE: RNA

James,
I have used the following procedure. The probe binds to nucleoli in the cells if the mRNA is being synthesised by the cell.

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html

SOLUTIONS:


1.      Acetic Alcohol
                Absolute Alcohol        15ml
                Acetic Acid                   5ml

2.      Absolute Alcohol

3.      Cold Acetone


METHOD:

1. Air dry frozen sections (6-8um) minimum 1 hour.

2. Fix in Acetic Alcohol   15 minutes.

3. Wash in absolute alcohol.

4. Place in cold acetone, allow to warm to room temperature.

5. Remove slides and air dry.

6. Circle sections with PAP pen, add probe and continue with ISH.

-----Original Message-----
From: james.zimmerman@pharma.novartis.com
[mailto:james.zimmerman@pharma.novartis.com]
Sent: Thursday, 21 November 2002 9:06
To: Tony Henwood
Subject: RE: RNA



What type fixative do you use and for how long ?

                        JPZ



**********************************************************************
This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you are not the intended recipient, please
delete it and notify the sender.

Views expressed in this message and any attachments are those
of the individual sender, and are not necessarily the views of the
Childrens Hospital at Westmead

This footnote also confirms that this email message has been
virus scanned and although no computer viruses were detected,
the Childrens Hospital at Westmead accepts no liability for any
consequential damage resulting from email containing computer
viruses.
**********************************************************************

<< Previous Message | Next Message >>