From:Tony Henwood


I have used the following procedure. The probe binds to nucleoli in the cells if the mRNA is being synthesised by the cell.

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318


1.      Acetic Alcohol
                Absolute Alcohol        15ml
                Acetic Acid                   5ml

2.      Absolute Alcohol

3.      Cold Acetone


1. Air dry frozen sections (6-8um) minimum 1 hour.

2. Fix in Acetic Alcohol   15 minutes.

3. Wash in absolute alcohol.

4. Place in cold acetone, allow to warm to room temperature.

5. Remove slides and air dry.

6. Circle sections with PAP pen, add probe and continue with ISH.

-----Original Message-----
Sent: Thursday, 21 November 2002 9:06
To: Tony Henwood
Subject: RE: RNA

What type fixative do you use and for how long ?


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