Hermina

If you weren't so far away I would send you some Cajeput.  However, I would
dispute the need for the Cajal's.  Beautiful it certainly is but the use of
mercuric chloride puts it beyond the pale.  If you do use it ignore Cajeput
as conventional DCM works pefectly well.  My method of choice is
immunochemistry.  Simple and with a selection of easily available
antibodies.  A good PTAH demonstrate astrocytes well but, for obvious
reasons, only outside white matter.

Thank you for reminding me about Cajal's.  It was one of the few frozen
techniques I really enjoyed.   

Andy Shand     

-----Original Message-----
From: Hermina Borgerink [mailto:hborgeri@wfubmc.edu]
Sent: 27 November 2002 20:46
To: histonet
Subject: Oil of cajeput


Good afternoon histonetters,
 
I have been asked to stain for astrocytes, using the antibody for GFAP
(Glial Fibrillary Acidic Protein), as well as the actual stain.  There are
various procedures in Sheehan and Hrapchak for the demonstration of
astrocytes and their processes using parrafin sections in addtition to the
gold standard: Cajal's gold sublimate method using frozen sections.
However, if possible, I would prefer to start of with paraffin sections.
But there is a slight problem:  both methods call for clearing in oil of
cajeput, and in my 40+ years in histology, I have never heard of this
clearing agent.  I would appreciate any information anyone may have to
enlighten me.
 
Thanks in advance and may you all have a wonderful and peaceful Thanksgiving
holiday.
Hermina
 
 
Hermina M. Borgerink, BA, HTL, HT(ASCP)IHQ
Department of Pathology
Wake Forest University School of Medicine
Medical Center Blvd
Winston-Salem, NC 27157
Phone (336)716-1538
Fax (336)716-1515
hborgeri@wfubmc.edu
 

Hermina M. Borgerink, BA, HTL, HT(ASCP)IHQ
Department of Pathology
Wake Forest University School of Medicine
Medical Center Blvd
Winston-Salem, NC 27157
Phone (336)716-1538
Fax (336)716-1515
hborgeri@wfubmc.edu