I have started using a double labeled Immuno, for slow and fast fibre types.
You can distinguish Type 1, 2a and 2b all in one stain on FFPE sections. The
procedure is from Journal of Clinical Pathology 2002;55:375-380, title "
Validation of a simple, rapid, and economical technique for distinguishing
type1 and 2 fibres in fixed and frozen skeletal muscle". The stain is really
beautiful! Let me know if you would like my protocol.

Mary Georger
Center for Cardiovascular Research
MRB 2-9816
585-273-1548

> ----------
> From: 	Ian Montgomery
> Sent: 	Tuesday, November 26, 2002 10:19 AM
> To: 	histonet@pathology.swmed.edu
> Subject: 	Muscle histochemistry.
> 
>         What's the current thinking on a palette of histochemical
> reactions for fibre typing. I'm still ATPase., dehydrogenases etc. Plus,
> immunohistochemistry, any suggestions here, in the past I simply used fast
> and slow myosin.
> Ian.  
> 
> 
> Dr. Ian Montgomery,
> Histotechnology,
> Graham Kerr Building,
> Institute of Biomedical & Life Sciences,
> University of Glasgow,
> Glasgow,
> G12 8QQ.
> Tel: 0141 339 8855 
> Office: 4652
> Lab: 6644.
> e-mail: ian.montgomery@bio.gla.ac.uk
>