RE: Mouse sectioning

Hi Patti and other folks working with mice:
At the P1 time point I usually bisect the head, fix in 4% Paraformaldehyde for 3 hours.  No decal this early (I decal at P12 and up).  Section at 4-5 um in paffin.  Hope this helps.  Maureen @ East Carolina U.

-----Original Message-----
From: Patti Loykasek <>
To: histonet <>
Sent: Thu, 21 Nov 2002 13:02:04 -0800
Subject: Mouse sectioning

I have a question about processing and sectioning mouse tissue. We have been
asked about processing, embedding, and sectioning day old mouse heads. We
will need to have the eye and ear in the same plane. I would like some input
on fixation times, decal or no decal, processing schedules, paraffin
preferences, and any other insights anyone would like to share. Thank you
for the help, as always.

Patti Loykasek
Phenopath Laboratories
Seattle, WA

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