RE: Astrocytes-Special stain

From:"Dawson, Glen"

GFAP from DAKO (cat. # M0761) works well on human astrocytoma.

No antigen retrieval required.  LSAB2 (DAKO) detection.

Good Luck,

Glen Dawson

-----Original Message-----
From: Geoff McAuliffe [mailto:mcauliff@UMDNJ.EDU]
Sent: Thursday, November 21, 2002 2:18 PM
To: Jo Dee Fish
Subject: Re: Astrocytes-Special stain

An immuno for GFAP (glial fibrillary acidic protein) works well on
formalin-fixed, paraffin embedded rat and mouse brain (using DAKO rabbit
anti-cow antibody and Vector ABC kit), should be worth a try on human


Jo Dee Fish wrote:

> Hello Histonetters,
> Does anyone know of a special stain for astrocytes.  This would be
> for paraffin embedded brain.
> I know of Cajal's stain, but unfortunately it is for frozen sections.
> Thanks much,
> Jo Dee
> #003#Jo Dee Fish
> Research Technologist II
> Gladstone Institue of Cardiovascular Disease
> Telephone: (415) 695-3720
> Fax: (415) 285-5632
> E-mail:
> Mailing address:
> Gladstone Instutes
> P.O. Box 419100
> San Francisco, CA 94141-9100

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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