Paraffin processing BCIP/NBT stained embryos

From:"Johnson, Teri"

Hi again,

I have been asked to paraffin process some embryos which have had ISH done on them using BCIP/NBT as the substrate.  How stable is this chromogen for this process?  I suspect some of it will leach out in the alcohols.  I use Pro-Soft (propanol, propylene glycol ether, propylene glycol ester) to dehydrate and aliphatic hydrocarbon to clear.  I'm hoping this will help minimize chromogen loss.  Additionally I plan to limit the amount of time in the dehydration stages.

Got any suggestions?


Teri Johnson
Managing Director Histology Core Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110

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