Good afternoon histonetters,
 
I have been asked to stain for astrocytes, using the antibody for GFAP (Glial Fibrillary Acidic Protein), as well as the actual stain.  There are various procedures in Sheehan and Hrapchak for the demonstration of astrocytes and their processes using parrafin sections in addtition to the gold standard: Cajal's gold sublimate method using frozen sections.  However, if possible, I would prefer to start of with paraffin sections.  But there is a slight problem:  both methods call for clearing in oil of cajeput, and in my 40+ years in histology, I have never heard of this clearing agent.  I would appreciate any information anyone may have to enlighten me.
 
Thanks in advance and may you all have a wonderful and peaceful Thanksgiving holiday.
Hermina
 
 
Hermina M. Borgerink, BA, HTL, HT(ASCP)IHQ
Department of Pathology
Wake Forest University School of Medicine
Medical Center Blvd
Winston-Salem, NC 27157
Phone (336)716-1538
Fax (336)716-1515
hborgeri@wfubmc.edu
 

Hermina M. Borgerink, BA, HTL, HT(ASCP)IHQ
Department of Pathology
Wake Forest University School of Medicine
Medical Center Blvd
Winston-Salem, NC 27157
Phone (336)716-1538
Fax (336)716-1515
hborgeri@wfubmc.edu