Help using osteobed and histocryl
|From:||Lenaldo Branco Rocha |
I've been trying to using Osteobed resin to embed small calcified bone
samples. However I've got problems with the polymerization step, the resin
becomes a rubber-like mass. I tried the manufacturer's protocol and few
variations for methylmetacrilate found in literature but none of them
worked. Any hint?
And regarding Histocryl, has anyone tried any immnuhistochemical or
histoenzymology protocol in tissues embedded in it? Does it need the removal
of resin prior the reaction?
I would be grateful with any help.
<< Previous Message | Next Message >>