GFP paraffin sections and/or =?UNKNOWN?Q?=DF-galactosidase?=
one question I have:
Does anybody has a protocol for immunostaining with
anti-gfp in paraffin embedded tissues/sections, we
have very low repoter expression and are not able to
see fluorescency by microscopy.
Or is there the other possibility with #223#-gal as
reporter: I thought of fixing the whole organism
(small snails) and incubate them in #223#-gal staining
solution before embedding in paraffin and section..
Thank you very much for help !
Institute of Molecular Parasitology
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