From: | Carlos Defeo |
Dear Yan: Muscle is a tissue that deserves special attention. If you like to cut it better I suggest you to use methyl salycilate as clearant agent,you can let pieces stand overnight. You can do a last bath in toluene for extracting some of the salycilate, and then prolong the baths in paraffin doing many changes, this can take many hours without hardening excessively the pieces. Good luck, Carlos.Histotechnologist,Montevideo,Uruguay